【摘要】 利用SDS-PAGE和Western blotting证明了MCF-7细胞中组蛋白H3 Thr 3磷酸化是有丝分裂期特异性的.间接免疫荧光标记和激光共聚焦显微镜显示H3 Thr 3磷酸化起始于MCF-7细胞早前期的整个核中.事实表明,这种整体性的磷酸化是有丝分裂期间染色体整体性的变构凝集所必需的.中期,以点状分布的Thr 3磷酸化的组蛋白H3可能参与动原体的组成以促进子代染色体的分离.后期,H3 Thr 3磷酸化的信号变弱并趋于在染色体上均匀分布,当细胞进入有丝分裂末期及胞质分裂期H3 Thr 3磷酸化的荧光信号完全消失.所有结果表明在有丝分裂期间组蛋白H3的磷酸化各自发生在不同位点,执行不同的功能.更多还原

【Abstract】 We demonstrated using SDS-PAGE and Western blotting that histone H3 Thr 3 was phosphorylated specially during·mitosis in MCF-7 cells.Indirect immunofluorescence labeling and laser confocal microscopy showed that H3 Thr 3 phosphorylation initiated in the whole nuclear at early prophase in MCF-7 cells. The evidence indicated that the entire phosphorylation is required for the entire remodeling and condensation of chromosomes during mitosis.At metaphase,Thr 3 phosphorylated H3 presented as punctate distribution may participate in the active kinetochore to promote filial generation chromosome segregation.The signals of H3 Thr 3 phosphorylation were faint and tended to distribute homogeneously at chromosome in anaphase.When cells proceeded to telophase and cytokinesis,the fluorescence signals of H3 Thr 3 phosphorylation vanished completely. All the results suggested that the phosphorylation of histone H3 occurs at different sites respectively and plays different roles during mitosis.更多还原