【摘要】 通过简并PCR和TAIL-PCR技术从瓶霉Phialophora sp. G5菌株基因组DNA中克隆得到一个新的编码b-1,4-木聚糖酶的基因,命名为xyn11G5。该基因ORF全长879 bp,共编码292个氨基酸和一个终止密码子,前19个氨基酸为信号肽序列。将xyn11G5在毕赤酵母中进行分泌表达,重组蛋白经纯化达到电泳纯。酶学性质分析表明,该重组木聚糖酶最适温度为50℃,最适pH为5.0,在中性条件下具有良好的稳定性,并且具有一定的热稳定性,在饲料行业和生物能源行业领域具有潜在的应用前景。更多还原

【Abstract】 A xylanase gene xyn11G5 was cloned from Phialophora sp.G5 by degenerate PCR and TAIL-PCR method.Its ORF was 879 bp,encoded 292 amino acids residues,and the frontal 19 residues composed a putative signal peptide.Xyn11G5 was successfully expressed in Pichia pastoris and purified.The enzymatic characteristics analysis showed that the optimum pH and temperature for the recombinant xylanase was pH 5.0 and 50℃,respectively.It showed pH stability under neutral and thermo stability.Xyn11G5 was a good candidate in animal feed and bio-fuel industrial application.更多还原