【摘要】 目的:检测各种脂肪细胞因子在大鼠急性胰腺炎中的表达及其可能意义。方法:40只SD大鼠随机分为4组:正常对照组、假手术组、急性水肿型胰腺炎(AEP)组和急性坏死型胰腺炎(ANP)组,AEP组腹腔注射雨蛙素,ANP组腹腔注射精氨酸,正常对照组不予以任何处理,假手术组给予同等剂量的生理盐水腹腔注射,应用ELISA法测定大鼠血清中抵抗素、瘦素、脂联素、肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β水平的变化,同时检测血清中淀粉酶(AMY),计算胰腺/体重比值,观察胰腺组织病理变化。结果:AEP组和ANP组血清淀粉酶、胰腺/体重比值、胰腺组织病理评分、血清抵抗素、瘦素、TNF-α、IL-1β水平均较对照组和假手术组明显升高,两者之间差异显著(P<0.01);ANP组较AEP组也升高,两者之间有统计学意义(P<0.01或P<0.05);而AEP组中脂联素水平为(3.26±0.68)mg/L,ANP组中为(2.27±0.45)mg/L,与正常对照组和假手术组比较明显降低,且ANP组较AEP组也降低,统计学上差异显著(P<0.01或P<0.05);血清抵抗素水平与TNF-α、IL-1β水平、胰腺组织病理评分呈正相关,相关系数r为0.711、0.794和0.812(P<0.01);血清瘦素水平与TNF-α、IL-1β水平、胰腺组织病理评分呈正相关,相关系数r为0.736、0.812和0.785(P<0.01或P<0.05),而血清脂联素与TNF-α、IL-1β水平、胰腺组织病理评分呈负相关,相关系数r为-0.796、-0.809、-0.788(P<0.01或P<0.05)。结论:脂肪细胞因子与急性胰腺炎发病发展有关,有可能成为预测急性胰腺炎严重性和预后的有用指标之一,其中抵抗素、瘦素的增加可能加重急性胰腺炎病情的进展,而脂联素增加可能起到保护作用,为急性胰腺炎的治疗提供了新的靶点。更多还原

【Abstract】 Objective: To investigate the change and potential significance of adipokine in the rat of acute pancreatitis. Methods:Forty Sprague-Dawley rats were randomly divided into four groups: control group,sham operation group,acute edematous pancreatitis (AEP) group and acute necrotizing pancreatitis (ANP) group. AEP model was induced by intraperitoneal injection of cerulein and ANP model was induced by intraperitoneal injection of L-arginine while the control group didn’t receive anything,the sham operation group received same volume of 0.9% normal saline injected. The level of amylase,resistin,leptin,adiponectin,TNF- ,IL-1β in rats blood serum were determined by ELISA,and pancreas/body weight ratio was evaluated and pancreatic pathology was observed. Results: The levels of amylase,pancreas/body weight ratio,pancreatic pathology,resistin,leptin,TNF- and IL-1β in AEP group and ANP group were significantly higher than those in the control group and the sham operation group(P < 0.01),which in ANP group were higher than those in AEP group (P < 0.01 or P < 0.05). The levels of adiponectin in both AEP and ANP group were (3.26 ± 0.68),(2.27 ± 0.45) mg/L,respectively,which were significantly lower than those in the control and sham operation group(P < 0.01),it was lower in ANP group than in AEP group (P < 0.05). The level of resistin was correlated with the increase of TNF- ,IL-1 and the pathologic severity of pancreatitis(r = 0.711,0.794,0.812,respectively,P < 0.01). The level of leptin was correlated with the increase of TNF- ,IL-1β and the pathologic severity of pancreatitis(r = 0.736,0.812,0.785,respectively,P < 0.01 or P < 0.05). But the level of adiponectin was correlated with the decrease of TNF- ,IL-1β and the pathologic severity of pancreatitis(r = -0.796,-0.809,-0.788,respectively, P < 0.01 or P < 0.05). Conclusion: The adipokine was correlated with the pathogenesis and development of acute pancreatitis. The adipokine may be useful to predict the severity and prognosis of acute pancreatitis. The increased levels of resistin and leptin may act to aggravate the severity of acute pancreatitis. But the increased levels of adiponectin may act to protect the severity of acute pancreatitis.更多还原