【摘要】 研制了基于酪氨酸酶和氧化石墨烯修饰丝网印刷电极的生物传感器,并用于水中邻苯二酚的测定.1-芘丁酸琥珀酰亚胺酯分子通过非共价的π-π堆积作用吸附到氧化石墨烯上,酪氨酸酶与1-芘丁酸琥珀酰亚胺酯形成酰胺键,组装到氧化石墨烯上,制得纳米复合材料.该材料为酪氨酸酶的固定提供了有利的微环境,并且能很好地保持其生物活性.传感器对邻苯二酚响应灵敏、快速,达到稳态电流时间在9 s以内.实验结果表明,传感器的重现性、选择性和稳定性良好,检测线性范围为8.1×10-8 mol/L～2.2×10-5 mol/L,检出限为4.7×10-8 mol/L.所研制的传感器可应用在水中酚类化合物的现场快速检测之中.更多还原

【Abstract】 A disposable biosensor has been developed for the determination of catechol in water based on tyrosinase-graphene oxide modified screen printed electrode.Graphene oxide sheets(GS) were noncovalently-functionalized with 1-pyrenebutanoic acid and succinimidyl ester(PANHS).Subsequently,the tyrosinase could covalently bind with PANHS-functionalized GS by forming amide bond.The as-prepared biocompatible nanocomposite could provide a biocompatible microenvironment to protect the activity of tyrosinase effectively.This disposable biosensor exhibited a fast amperometric response(less than 9 s),and a high sensitivity for monitoring catechol with good repeatability,selectivity and stability.The method showed good linearity in the range from 8.1×10-8 mol/L to 2.2×10-5 mol/L catechol with a detection limit of 4.7×10-8 mol/L.The results showed that the as-prepared disposable biosensor could be used in the rapid and in-situ monitoring of phenolic compounds in water samples.更多还原