【摘要】 氧化还原蛋白质和电极之间的直接电子转移在理论和实验上都具有重要的意义.通过对蛋白质和电极的修饰能够促进它们之间的直接电子转移.蛋白质与电极表面直接接触易导致蛋白质变性,在电极表面固定沸石或介孔分子筛可为氧化还原蛋白质提供一个类似于天然状态的微环境,通过分子筛的传输通道加速直接电子转移.本文主要介绍在沸石和介孔分子筛上固定化的蛋白质的电子转移和生物传感,并重点评述这些方面的研究进展、遇到的挑战及发展趋势.更多还原

【Abstract】 Direct electron transfer between redox proteins and electrodes is of practical and theoretical interest and can be improved by electrode or protein modification.The direct contact of protein with electrode surfaces can lead to a significant change of the protein structure and/or function.Immobilized microporous and mesoporous molecular sieves on electrode surfaces provides a microenvironment similar to that of the redox protein in native systems and gives the protein molecules more freedom in orientation,thus facilitating the direct electron transfer through the conducting tunnels of molecular sieves.This brief review focuses on the microporous and mesoporous molecular sieves used for protein immobilization,electron transfer and biosensing,with emphasis on recent advances,challenges and trends.更多还原