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地衣芽孢杆菌固态发酵代谢产物分析
Analysis of the Metabolites of Bacillus licheniformis by Solid Fermentation
【摘要】 从茅台酒生产环境中分离纯化得到1株细菌MTDB-02,经16S rDNA测序比对,最终将MTDB-02定性为地衣芽孢杆菌。将该菌在制曲工艺、堆积工艺以及兼性条件下分别进行纯种固态发酵,利用GC-MS对其代谢物进行检测解析。结果表明,该菌纯种固态发酵能代谢产生70多种物质。这些物质中有多种是食品中的风味物质和风味前体物质,亦是茅台酒呈香呈味物质中的一个组成部分。
【Abstract】 MTDB-02,was easily isolated from the production environment of Moutai Liquor.MTDB-02 was identified as Bacillus licheniformis by 16S rDNA identification.Then pure solid fermentation of MTDB-02 was carried out under starter-making conditions,stacking conditions,and facultative conditions respectively and GC-MS was applied to anlayze its metabolites.The results showed that MTDB-02 could produce more than 70 metabolites by pure solid fermentation.Most of these metabolites were flavoring compounds and flavoring precursors for food,and also an integral part of aroma-producing & flavoring-producing substances in Maotai Liquor.
【Key words】 microbe; Bacillus licheniformis; solid fementation; metabolites; Maotai flavor;
- 【文献出处】 酿酒科技 ,Liquor-Making Science & Technology , 编辑部邮箱 ,2011年09期
- 【分类号】TS261.1
- 【被引频次】15
- 【下载频次】654