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水杨酸对废电池胁迫下绿豆幼苗抗氧化酶活性及生理特性的影响

Effect of Salicylic Acid on Antioxidant System of Mungbean(Vigna radiata) Seedling Under Used Batteries Stress

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【作者】 王红星纪秀娥陈晓君尚燕史留功

【Author】 WANG Hong-xing,JI Xiu-e,CHEN Xiao-jun,SHANG Yan,SHI Liu-gong(College of Life Science,Zhoukou Normal University,Zhoukou 466000,China)

【机构】 河南省周口师范学院生命科学系

【摘要】 采用室内有培养土的纸杯培养方法,研究了不同浓度水杨酸(SA)处理对废电池胁迫下,绿豆幼苗抗氧化酶及生理特性的影响。结果表明,废电池胁迫下,超氧化物歧化酶(SOD)活性升高,过氧化物酶(POD)活性降低,光合色素、可溶性蛋白含量下降,脯氨酸和丙二醛(MDA)含量升高,电导率增大,膜稳定性降低,显示出一定的毒害效应;低浓度(≤100mg·L-1)的外源SA处理能够明显增强废电池胁迫下绿豆叶片SOD、POD活性,改善多项指标,但随着SA浓度升高,SOD和POD酶活性逐渐降低。说明低浓度SA能通过刺激抗氧化酶活性,减轻氧化胁迫,缓解废电池对绿豆幼苗的毒害作用,但高浓度SA(≥100mg·L-1)缓解作用降低。

【Abstract】 This paper investigated the effect of different treatments of salicylic acid(SA) on antioxidant enzyme activities and physiological characteristics of mungbean seedling under used batteries stress.The results showed that used batteries regulated up the superoxide dismutase(SOD) activities of mungbean seedling and its conductivity,down the Peroxidase(POD) activities,with decreased contents of photosynthetic pigments and soluble proteins,and increased contents of MDA and proline.The data of physiological characteristics indicated that the mungbean seedling was injured with the membrane system damaged.The treatment with low concentration of SA(≤100 mg·L-1) promoted the activities of SOD and POD,and improved many index of seedling under the used batteries stress significantly.When the SA concentration over 100 mg·L-1 the activities of SOD and POD began to turn down with the increase of SA concentration.It was suggested that used batteries stress on mungbean seedling could be relieved by activating antioxidant enzyme activities and suquently reducing oxidative stress under the treatment of SA,but the relieving of high concentration of SA was lowered.

【关键词】 绿豆废电池水杨酸抗氧化酶
【Key words】 Vigna radiataused batteriessalicylic acidantioxidant enzyme
  • 【文献出处】 农业环境科学学报 ,Journal of Agro-Environment Science , 编辑部邮箱 ,2011年03期
  • 【分类号】X173
  • 【被引频次】20
  • 【下载频次】264
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