【摘要】 采用PCR产物直接测序法测定了2尾来自中国南海的日本竹筴鱼(Trachurus japonicus)线粒体DNA控制区基因全序列,并结合从GenBank中下载的8种竹筴鱼属相应序列,对竹筴鱼属控制区序列进行了结构分析,识别出终止序列区、中央区和保守序列区3个区域,并找到了2个与DNA复制终止相关的序列TAS和中央保守区的保守序列CSB-F、CSB-E和CSB-D,以及保守序列区的保守序列CSB1、CSB2和CSB3。以鳜(Sinipercachuatsi)作为外群,使用邻接法和最大简约法构建了9种竹筴鱼属的系统发育树。竹筴鱼属鱼类为单系类群,蓝竹筴鱼(T.picturatus)、智利竹筴鱼(T.murphyi)、太平洋竹筴鱼(T.symmetricus)、南非竹筴鱼(T.capensis)和竹筴鱼(T.trachurus)构成一支,地中海竹筴鱼(T.mediterraneus)、青背竹筴鱼(T.declivis)、日本竹筴鱼(T.japonicus)和新西兰竹筴鱼(T.novaezelandiae)为另一支;日本竹筴鱼未单独成一支,而是聚入青背竹筴鱼和新西兰竹筴鱼之间,初步猜测日本竹筴鱼和新西兰竹筴鱼为同种异名。更多还原

【Abstract】 We used PCR to amplify the complete sequences of mitochondrial DNA(mtDNA) control region of 2 Trachurus japonicus from South China Sea,and then aligned them by Clustal X with 8 other species of the genus Trachurus from GenBank.According to the alignment and structure comparison,the mtDNA control region of the genus Trachurus can be divided into 3 domains: termination associated sequence domain(TAS),central conserved domain(CD) and conserved sequence block domain(CSB).We identified 2 termination-associated sequences(TAS) and 3 conserved blocks(CSB-F,CSB-E and CSB-D) in CD domain as well as 3 conserved sequence blocks(CSB-1,CSB-2 and CSB-3) in CSB domain.Taking Siniperca chuatsi as outgroup,the molecular phylogenetic relationship among 9 Carangidae was analyzed using neighbor-joining(NJ) method and maximum parsimony method in MEGA 4.0.Results show that the genus Trachurus fishes belong to a monophyletic group: T.picturatus,T.murphyi,T.symmetricus,T.capensis and T.trachurus gather into one cluster;T.mediterraneus,T.declivis,T.japonicus and T.novaezelandiae gather another.T.japonicus does not form into an independent group but is merged into T.declivis and T.novaezelandiae.Thus,it is suggested that T.japonicus and T.novaezelandiae may be 2 synonyms.更多还原