【摘要】 通过采用60Co-γ辐照处理稻草秸秆,提高稻草秸秆的酶解效果。采用离子色谱仪测定稻草秸秆酶解液中可发酵性糖的含量,对水解温度、加酶量、液固比、水解时间4个因素进行单因素试验分析,对稻草秸秆酶解条件进行优化,建立稻草秸秆辐照酶解新工艺。研究结果表明,稻草秸秆辐照剂量在0～2 000 kGy内,辐照预处理最佳剂量为1 200 kGy;得到稻草秸秆最优辐照酶解条件:预处理辐照剂量为1 200 kGy、水解温度为45℃、水解时间为36 h、液固比为60、加酶量为120 U/g。在此最佳条件下,稻草秸秆纤维素、半纤维素总转化率达71%。更多还原

【Abstract】 In this paper,rice straw was treated by 60 Co-γ irradiation and cellulase to improve the enzymatic hydrolysis efficiency.Fermentable sugars in the rice straw enzymes-hydrolyzed liquid were measured by Ion Chromatography.Hydrolysis temperature,enzyme dosage,liquid-to-solid ratio and hydrolysis time was analyzed respectively,the optimized enzyme hydrolysis conditions were obtained by orthogonal experiments.The results showed that the optimal irradiation dose was 1 200 kGy when the range form 0 to 2 000 kGy.And the optimum conditions of enzyme hydrolysis was 50 ℃,36 h,liquid-to-solid ratio:60,enzyme dosage:120 U/g.The total conversion of cellulose and hemicellulose up to 71%.更多还原