【摘要】 根据肉毒梭菌神经毒素基因设计的引物和探针,建立了肉毒梭菌荧光定量PCR快速检测方法。肉毒梭菌产生了典型的"S"型曲线,其他干扰菌都无扩增,说明引物特异性较好,检测灵敏度为1×103 cfu/mL。该方法能够实现肉毒梭菌的快速检测,具有灵敏度高、特异性好的特点。更多还原

【Abstract】 According to the botulinum neurotoxin gene,we establish fluorescent real-time PCR method for the rapid testing of Clostridium botulinum.The primers are highly specific,with a typical S shaped curve produced for Clostridium botulinum in the fluorescent real-time PCR,while the interfering bacteria do not amplify.The sensitivity of fluorescence real-time PCR for Clostridium botulinum is 1×10 3 cfu/mL.This method is able to achieve rapid testing of Clostridium botulinum with a high specificity and sensitivity.更多还原