【摘要】 试验用菌株是分离自山西省右玉县的野生种,经过多次分离纯化,发现该菌株在PDA培养基上的长势缓慢且菌丝稀疏,极易老化等。结合现有关文献资料,对其菌丝培养基进行了优化筛选,旨在对其生长及液体培养生长机理的进一步研究奠定基础。以菌丝长速和菌丝干质量为测量指标,结合菌丝长势情况,筛选出了适合该菌株生长的基础培养基、碳源、氮源、碳氮比和无机盐,并进一步正交试验优化,得到了该菌株的最佳培养基。更多还原

【Abstract】 The wild strain is isolated from Agaricus arvensis of Youyu county in Shanxi province,and it has been separated and purificated for several times,But the mycelial has poor growth and rapid aging easily.The experiment refers to literature materials on hand,the culture conditions of the strain are optimized in order to further improve its growth and laid foundation for the mechanism of its liquid culture.Using mycelial growth rate and mycelial dry weight as indexes,the optimal basic medium,carbon source,nitrogen source,suitable C/N and lnorganic salt are selected,and then further optimized by a L16(43)orthogonal experiment.The results show that optimum conditions are ameliorated CYM medium.更多还原