【摘要】 目的:观察以富含血小板血浆(platelet-rich plasma PRP)介导的双相接种法构建的组织工程骨在体内的成骨是否优于普通双相接种法和传统静滴接种法。方法:用传统静滴法(对照组)和富含血小板血浆(PRP组)、乏血小板血浆(PPP组)双相接种法构建组织工程骨,并用其修复山羊颅骨临界缺损,术后3月通过CT扫描、组织病理学观察三组动物颅骨缺损处成骨情况。结果:PRP组的成骨量显著较PPP组合和对照组(P<0.05),PPP组合和对照组无显著差异(P>0.05)。结论:用PRP介导的双相接种法构建组织工程骨在体内早期成骨优势显著;PRP双相接种法是安全、简便、实用、高效的细胞接种方法。更多还原

【Abstract】 Objective To confirm whether platelet-rich plasma(PRP) biphasic seeding strategy can significantly enhance the osteogenesis in vivo,compared with common biphasic seeding and traditional dropping seeding.Methods To construct tissue engineering bones with PRP biphasic seeding,common biphasic seeding and traditional dropping seeding respectively.To repair the goat skull clinical defects with the three groups of tissue engineering bones.At 3 days and 3 months postoperatively,goat skulls were examined by CT scanning,the pictures were analyzed by the Osirix software.Following CT scanning at 3 months postoperatively,animals were executed,the skull samples were harvested and examined histologically.Results The new bone amount of the group of PRP biphasic seeding was significantly more than the groups of common biphasic seeding and traditional dropping seeding(P<0.05).The difference between the common biphasic seeding group and the traditional dropping seeding group was not obvious(P>0.05).Conclusion The PRP biphasic seeding was safe,efficient and practical.The tissue engineering bone constructed by the PRP biphasic seeding strategy can improve the osteogenesis in vivo.更多还原