【摘要】 目的探讨神经生长因子(NGF)对2,5-已二酮(2,5-HD)诱导的大鼠中毒性周围神经病的疗效。方法大鼠给予2,5-HD间隔灌胃37d成功诱导模型后,肌肉注射NGF1000、2000、4000 BU/(g.d),治疗11d,正常组和模型对照组给予相同剂量的生理盐水,评估神经病体征损伤评分(姿态、步态和肌力变化)、后肢电生理学检查和坐骨神经的组织病理学检查。结果与正常组比较,2,5-HD模型对照组大鼠的肢体运动功能明显恶化,神经-肌肉动作电位潜伏期显著延长,动作电位幅度显著降低。2,5-HD中毒引起大鼠坐骨神经的组织病理学改变,主要包括轴突的肿胀变性和髓鞘壁的肿胀,NGF肌肉注射后可促进大鼠肢体运动功能的恢复、缩短因中毒所致大鼠坐骨神经-肌肉电潜伏期的延长、纠正神经-肌肉动作电位幅度下降、逆转神经轴索变性、加速损伤神经纤维功能和形态的复原。结论NGF肌肉注射可明显改善2,5-HD诱发的大鼠中毒性周围神经病,为临床应用NGF治疗正己烷中毒性神经病提供理论依据。更多还原

【Abstract】 Objective To observe the effects of mouse nerve growth factor(NGF)on 2,5-hexanedione (2,5-HD)induced toxic peripheral neuropathy in rats.Methods Rats were administered different doses of NGF 1000,2 000,and 4 000 BU/(kg·d)intragastrically for 11 days after peripheral neuropathy had been induced by intermittent exposure to 2,5-HD for 37 days.Normal control group and model group were given the same amount of physiological saline.The scores of neuropathic signs(gesture,gait and muscular strength abnormality),electrophysiological in the hind limb and histopathological examinations of the sciatic nerves were evaluated.Results When compared with normal control group,rats in 2,5-HD induced group displayed neurobehavioral and electrophysiological responses significant deterioration but they were obviously ameliorated in NGF-treated rats.2,5-HD intoxication produced a histopathological change in tibial nerve fibers,including swelling,degeneration of axons,and myelin sheath swelling.Having been treated with NGF 2 000 or 4000 BU/(kg·d)for 11 days,the sheath swelling of tibial nerve fibers was obviously alleviated as compared with that in model group,and the morphology of axons was very close to that in normal rats.Conclusion NGF exerts a biologically significant neuroprotective effect against 2,5-HD induced peripheral neurotoxicity in rats.It provides evidence for clinical application of NGF to treat patients with n-hexane neuropathy.更多还原