【摘要】 目的:选择淋巴管特异内皮标志物LYVE-1在胃癌组织中的表达,探讨其淋巴管生成机制,及其与淋巴结转移和预后等临床病理参数的关系。方法:利用免疫组织化学方法检测LYVE-1在53例胃癌组织及10例正常胃黏膜组织中的表达,并进行微淋巴管密度(lymphatic vessel density,MLD)计数;结合临床病理、随访资料分析LYVE-1阳性MLD与胃癌淋巴结转移、预后等临床病理特征的关系。结果:在胃癌组织中,瘤周LYVE-1阳性MLD在淋巴结转移阳性组明显高于淋巴结转移阴性组(P<0.01);并显著高于正常胃黏膜组织及瘤内组织(P<0.01);后两者MLD无显著性差异(P﹥0.05)。而瘤内LYVE-1阳性MLD在各临床病理参数之间亦无显著差别(P﹥0.05)。单因素分析表明,瘤周MLD、淋巴结转移和病理分期是影响胃癌患者预后的因素(P<0.01);而进一步Cox模型多因素分析表明,瘤周MLD、淋巴结转移和病理分期是影响胃癌患者预后的独立危险因素(P<0.05),高MLD较低MLD预后差。结论:瘤周淋巴管对胃癌的发生、发展起重要作用,而瘤内淋巴管是无功能的。高瘤周淋巴管生成是胃癌患者的有意义的、独立的、不良预后因子。更多还原

【Abstract】 Objective:To select LYVE-1 the most specific lymphatic endothelial marker,for further studying the mechanisms of intra-and peritumoral lymphangiogenesis and the relevance to various clinical pathological parameters,including the presence of lymph node metastasis and prognosis in gastric cancer.Methods:The expression of LYVE-1 was quantified in 53 paraffin sections of gastric carcinoma and 10 gastric ulcer tissues using immunohistochemistry.Micro-lymphatic vessel density(MLD) was measured.The clinical significance was studied by analyzing the relationships of peritumoral and intratumoral lymphangiogenesis to various clinical pathological parameters and follow-up data in 53 cases of gastric carcinoma.Results:LYVE-1 positive MLD in peritumoral regions of gastric carcinoma tissues and in lymph node metastasis group was significantly higher than that in normal tissue and in no lymph node metastasis group(P﹤0.01).No significant difference between normal tissues and intratumoral regions was observed(P﹥0.05).Univariate analysis suggested that peritumoral MLD,the presence of lymph node metastasis and pathological stage were prognostic factors for gastric cancer patients(P﹤0.01).Multivariate analysis indicated that they were also independent prognostic factors in gastric cancer patients(P﹤0.05),and that high MLD was associated with a poor prognosis.Conclusion:Peritumoral lymphangiogenesis may play an important role in the onset and progression of gastric carcinoma,while intratumoral lymphangiogenesis may be no effect.High peritumoral lymphangiogenesis is a significant and independent factor indicating poor prognosis in gastric carcinoma patients.更多还原