【摘要】 目的白细胞介素(interleukin,IL)-6通过凝血酶及蛋白酶激活受体(proteinase activated receptor,PARs)的激动肽分别激发高度纯化后的单核细胞,分析人单核细胞分泌IL-6的诱发因素,为IL-6的临床试验提供参考。方法用凝血酶及PARs的激动肽分别激发高度纯化后的单核细胞16 h后,采用ELISA法检测单核细胞培养上清液中的IL-6。结果凝血酶呈浓度依赖性地诱导单核细胞产生IL-6,当凝血酶活性被抑制后,诱导单核细胞产生IL-6的能力被抑制。PAR-1激动肽SFLLR-NH2和PAR-4激动肽GYPGQV-NH2呈浓度依赖性地诱导单核细胞产生IL-6,PAR-1、PAR-4不能诱导单核细胞产生IL-6,而PAR-3激动肽TFRGAP-NH2和反激动肽PAGRFT-NH2对于单核细胞产生IL-6无明显作用。结论凝血酶可以诱导高度纯化的成人外周血单核细胞产生IL-6,其作用依赖于酶活性,说明它可能是通过PARs激活单核细胞。更多还原

【Abstract】 Objective By adjusting the cell functions,the inflammation and immune response interleukin-6(IL-6)play an important role.Using thrombin and proteinase activated receptor(PARs)peptide respectively to set a high degree of monocyte.Analysis of character reference of IL-6 by human monocytes,Il-6 for the clinical tests may be provided.Methods Highly purified monocytes were challenged for 16 hours with thrombin and agonist peptides of PARs,and measured the levels of IL-6 in culture supernatants of monocytes by ELISA.Results Thrombin provoked a dose dependent release of IL-6 from monocytes following 16 h incubation period.Thrombin inhibitor hirudin was able to inhibit thrombin induced secretion of IL-6 from monocytes.SFLLR-NH2,GYPGQV-NH2 stimulated increase in IL-6 secretion from monocytes following 16 h incubation,whereas its reverse peptide RLLFS-NH2 and VQGPYG-NH2 had little effects on IL-6 release.In contrast,TFRGAP-NH2 and its reverse peptide PAGRFT-NH2 showed little influence on IL-6 release from monocytes following 16 h incubation period.Conclusion Thrombin are potent stimuli of IL-6 secretion from highly purified monocytes.Their actions on monocytes depend on their enzymatic activities and are likely through activation of PARs.更多还原