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胃癌细胞SGC-7901对骨髓来源的间充质干细胞作用的研究
Interaction between bone marrow-derived mesenchymal stem cells and gastric carcinoma cell SGC-7901
【摘要】 目的建立胃癌细胞SGC-7901与骨髓来源的间充质干细胞相互作用的体外模型,检测其对间充质干细胞增殖、侵袭及迁移能力的影响,分析处于肿瘤微环境中的间充质干细胞生物学特性的改变。方法原代培养骨髓来源的间充质干细胞,传代培养胃癌细胞SGC-7901,建立间充质干细胞与胃癌细胞SGC-7901相互作用的体外模型,分析胃癌细胞SGC-7901对间充质干细胞增殖、侵袭及迁移能力的影响。结果 (1)胃癌细胞SGC-7901对间充质干细胞的增殖无影响(P>0.05)。(2)胃癌细胞SGC-7901可显著提高间充质干细胞的侵袭及迁移能力(P<0.05)。结论胃癌细胞SGC-7901对间充质干细胞有很强的趋化性,但对其增殖并无影响。
【Abstract】 Purpose An interaction model of gastric carcinoma cell SGC-7901 and bone marrow-derived mesenchymal stem cells(MSC) was established in vitro.The aim of this study was to detect the effect of SGC-7901 on proliferation and metastasis of MSC and to analyse the change of biological characteristics of MSC in tumor microenvironment.Methods MSC and SGC-7901 were cultured by primary culture and serial subcultivation.The interaction model was set up in vitro.The effect of SGC-7901 on proliferation,invasion and migration of MSC was analyzed by MTT and transwell inserts.Results SGC-7901 had no effects on the proliferation of MSC(P>0.05).SGC-7901 cells were able to promote the metastasis of MSC(P<0.05).Conclusion SGC-7901 cells can enhance metastasis but have no impact on proliferation of MSC in vitro.
【Key words】 gastric neoplasms； tumor microenvironment； bone marrow-derived mesenchymal stem cells； cell culture；
- 【文献出处】 临床与实验病理学杂志 ,Chinese Journal of Clinical and Experimental Pathology , 编辑部邮箱 ,2011年08期
- 【分类号】R735.2
- 【被引频次】6
- 【下载频次】164