【摘要】 目的检测环氧化酶-2(cyclooxygenase-2,COX-2)和诱导型氮氧合酶(inducible nitric oxidesynthase,iNOS)在结直肠癌(colorectal carcinoma,CRC)中的表达,为研究炎症与CRC发生的关系提供理论依据。方法应用流式细胞术和SP法免疫组化检测100例CRC及11例癌旁正常黏膜中COX-2和iNOS的表达,并结合临床病理因素进行分析。结果免疫组化显示,COX-2在CRC中表达阳性率为76.00%,而正常黏膜中只有9.10%,差异有显著性(P<0.05)。COX-2表达阳性率在CRC淋巴结转移组和无淋巴结转移组分别为87.50%(42/48)、65.38%(34/52),两组比较差异有显著性(P<0.05);FCM检测发现,高、中、低分化组平均FI值分别为2.47±1.41、2.70±1.08、3.01±1.26,各组COX-2的表达均高于正常黏膜组(1.00±0.28)(P<0.01);CRC淋巴结转移组(2.45±1.41)高于无淋巴结转移组(1.61±1.27)(P<0.05)。CRC中iNOS表达阳性率(81.00%)高于正常黏膜(27.27%,P<0.05),有淋巴结转移组的阳性率(91.67%)高于无淋巴结转移组(71.15%,P<0.05)。COX-2与iNOS表达呈正相关(P<0.05)。结论在CRC中COX-2和iNOS表达异常增高,在CRC中COX-2和iNOS的表达与淋巴结转移密切相关,可能在CRC的进展及转移中有重要作用。更多还原

【Abstract】 Purpose The purpose of this study was to detect COX-2,iNOS expression in colorectal carcinoma(CRC).This may provide theories for the research of inflammation and the colorectal carcinomas.Methods The expressions of COX-2,iNOS were studied in 100 cases of human colorectal carcinomas and 11 cases of normal colorectal mucosa by flow cytometry(FCM) and immunohistochemical staining.The clinical pathological significance of COX-2,iNOS expression in CRC were also analyzed.Results Immunohistochemistry showed that the positive rate of COX-2 expression in colorectal carcinoma was 76.00% while in normal mucosa was only 9.10%,the expression of COX-2 in two groups were significantly different(P<0.05).The positive rates in lymph node metastasis and without lymph node metastasis groups were 87.50%(42/48),65.38%(34/52) respectively.There was significant difference between the two groups too(P<0.05).The mean FI values of COX-2 expression detected by FCM in well,moderately and poorly differentiated groups were 2.47±1.41,2.70±1.08 and 3.01±1.26,respectively.The expression in each group was significantly higher than normal mucosa(1.00±0.28)(P<0.01).Also,COX-2 expression in lymph node metastasis group(2.45±1.41) was significantly higher than the group without lymph node metastasis(1.61±1.27)(P<0.05).The positive rate of iNOS expression in colorectal carcinomas(81.00%) was significantly higher than in the normal mucosa(27.27%)(P<0.05),and positive rate in lymph node metastasis group(91.67%) was significantly higher than the group without metastasis(71.15%)(P<0.05).Closely correlation was found between COX-2 and iNOS expression(P<0.05).Conclusions Expressions of COX-2,iNOS in colorectal carcinomas were abnormally increased and were closely related with lymph node metastasis.It may play an important role in the progression and metastasis of colorectal carcinoma.更多还原