【摘要】 目的:探讨白细胞介素-5(IL-5)及粒细胞-巨噬细胞集落刺激因子(GM-CSF)在哮喘发病过程中的作用及补脾益气中药对其含量的影响。方法:24只大鼠随机分成3组,每组8只,即:正常对照组、哮喘模型组和中药组。采用卵蛋白、灭活百日咳杆菌疫苗和氢氧化铝干粉配制的溶液腹腔注射致敏以及卵蛋白雾化吸入激发制备大鼠哮喘模型;正常对照组用等量生理盐水替代抗原液;中药组则胃饲补脾益气中药(加味玉屏风散)水溶液。观察支气管肺泡灌洗液(BALF)中IL-5及GM-CSF含量的变化。结果:与正常组比较,模型组BALF中IL-5及GM-CSF含量均显著增高(均P<0.05或P<0.01),尤其是IL-5增高更显著;相关分析表明,GM-CSF与IL-5、呈显著正相关(r1=0.519,r2=0.521,均P<0.05)。补脾益气中药能降低BAFL中IL-5及GM-CSF的含量(P<0.05或P<0.01)。结论:IL-5、GM-CSF在哮喘大鼠的气道炎症细胞浸润过程中起重要作用,可能加速了哮喘非特异性气道炎症的发生和发展;GM-CSF与IL-5之间存在密切的联系,可能对嗜酸粒细胞的聚集、迁移、活化和黏附有协同作用。补脾益气中药(加味玉屏风散)可以通过降低细胞因子水平来改善气道的炎症状况。更多还原

【Abstract】 Objective:To explore the role of IL-5 and GM-CSF in the course of asthmatic incidence,and the effect of the prescription and medicine of strengthening spleen and benefiting Vital Qi on content of both of them.Methods:Twenty-four male rats were randomly divided into three groups:normal control group;model group;Chinese Herbal group.Every group has eight rats.A sensitized asthmatic rat model was obtained by intraperitoneal injection egg albumin、inactivated Bordetella pertussis vaccine and aluminum hydroxide dry flour of prepared solution,and provocation by atomization breathe in egg albumin.Normal control group was supplied with partes aequales physiological saline to substitute antigen liquid;Chinese Herbal group was given prescription and medicine of strengthening spleen and benefiting Vital Qi(Add Ingredients Jade Screen Powder.The change of content of IL-5 and GM-CSF in broncho-alveolar lavage fluid(BALF) were observed.Results:Compared with normal control group,Model group BALF has dramatic growth of the content of IL-5 and GM-CSF(P<0.05 in all or P<0.01),especially the IL-5.Correlation analysis showed that GM-CSF and IL-5 were significantly direct correlated(r1=0.519,r2=0.521,P<0.05 in all).The prescription and medicine of strengthening spleen and benefiting Vital Qi can decrease the contents of IL-5 and GM-CSF in BALF(P<0.05 or P<0.01).Conclusion:IL-5 and GM-CSF play an important role in the course of the asthmatic rat`s air way inflammatory cell infiltration.They may accelerate the emergence and development of the nonspecific airway inflammation of asthma.There exists close link between IL-5 and GM-CSF,and it is expected that this has synergistic action on the aggregation,migration,activation and adherence of the eosinophil(EOS).The prescription and medicine of strengthening spleen and benefiting Vital Qi(Add Ingredients Jade Screen Powder) can improve the airway’s inflammation state through depressing the level of cell factor.更多还原