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正交试验法优选甜荞麦花叶中芦丁的提取工艺

Optimization of the Best Extraction Rutin Technology from Among the Gluco-buckwheat Floral Leaf by Orthogonal Experiment

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【作者】 王树华韩淑英耿菲张宏坡郝晨王猛周程艳

【Author】 WANG Shu-hua,HAN Shu-ying,GENG Fei,ZHANG Hong-po,HAO Chen,WANG Meng,ZHOU Cheng-yan(Basic Medical Department,North China Coal Medical College,Tangshan 063000,Hebei,China)

【机构】 华北煤炭医学院基础部

【摘要】 目的:优选甜荞麦花叶中芦丁的最佳提取工艺。方法:采用高效液相色谱技术,以荞麦花叶中所含的主要有效成分芦丁的含量为评价指标,采用L9(34)正交试验法,通过超声波辅助提取技术,考察了药材的粉碎度、所用溶剂、提取时间、提取次数四因素对荞麦芦丁提取率的影响。结果:药材为细粉、溶剂选用甲醇、提取时间60min、提取次数3次提取效果最好。结论:本实验为优化荞麦花叶中芦丁的提取工艺提供了参考依据。

【Abstract】 Objective:Optimizing of the best Extraction rutin technology from among the gluco-buckwheat floral leafMethods:By HPLC technology,taking the rutin content that the main effective component in the gluco-buckwheat floral leaf as evaluation index,adopt L9(34)orthogonal experiment,by supersonic wave assist Extraction technology,inspect the influence factors including comminution degree、solvent and number of timesResults:The best Extraction rutin technology is that medical material is fine powder,solvent is MeOH,Extraction time is 60 minutes,Extraction times is 3Conclusion:This experiment provided the best Extraction rutin technology from among the gluco-buckwheat floral leaf.

【基金】 河北省教育厅科技攻关项目(2006320);唐山市科技局科技攻关项目(10150204A-46);河北省自然科学基金项目(2010001795)
  • 【文献出处】 辽宁中医杂志 ,Liaoning Journal of Traditional Chinese Medicine , 编辑部邮箱 ,2011年06期
  • 【分类号】R284.2
  • 【被引频次】7
  • 【下载频次】227
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