【摘要】 目的分析老年汉族冠心病患者血凝素样氧化型低密度脂蛋白1(lectin-like oxidized low-density lipoproteinreceptor-1,LOX-1)501G/C和3’UTR位点C/T基因多态性与冠状动脉病变支数及狭窄程度的关系。方法选择行冠状动脉造影患者165例,根据冠状动脉造影结果分为冠状动脉正常对照组(对照组)38例,冠心病组127例。采用PCR单链构象多态PCR-SSCP法分析LOX-1501G/C和3’UTR位点C/T基因多态性。分析C基因型、T基因型与患者高敏C反应蛋白、纤维蛋白原的关系。结果 LOX-1第4外显子501G/C位点未见多态性表现。LOX-1 3’UTR位点可见C/T基因型。与对照组比较,冠心病组患者冠状动脉病变支数及冠状动脉狭窄程度均与3’UTR位点C/T基因发生频率,差异无统计学意义(P>0.05)。高敏C反应蛋白和纤维蛋白原水平在3’UTR位点C基因型与T基因型中差异无统计学意议(P>0.05)。结论 LOX-1基因多态性与汉族冠心病发病无关。更多还原

【Abstract】 Objective To study the relationship between polymorphism of lectin-like oxidized LDL receptor HLOX-1) gene and coronary artery disease in elderly Chinese Han population.Methods 165 subjects were divided into 2 groups according to results of coronary angiography:38 cases with normal coronary artery served as controls and 127 cases with coronary artery disease constituted coronary heart disease group.PCR single strand conformational polymorphism(PCR-SSCP) method was used to analyze LOX-1 501G/C and 3’UTR loci C/T gene polymorphism.The relationships of C genotype and T genotype with high sensitivity C-reactive protein and fibrinogen were analyzed.Results No polymorphism of Exon 4 was detected in all subjects.There was no significant difference in the 3’ UTR polymorphism between patients with coronary artery disease and controls.C/T genotype could be seen at LOX-1 3’UTR.Compared with control group.The number of diseased coronary arteries and degree of coronary stenosis had no statistically significant relation with difference in frequency of 3’UTR locus C/T gene.hs-CRP and fibrinogen levels had no statistically significant difference between C genotype and T genotype.Conclusions The results show that the LOX-1 polymorphism may be not associated with the risk of coronary artery disease in the Chinese Han population.更多还原