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南天竹组培快繁技术
A study on tissue culture of Nandina domestica
【摘要】 对彩色园林植物南天竹组培快繁技术进行研究,结果表明:MS+1.5 mg/L(质量浓度,后同)BA+0.1mg/L IBA是最佳的增殖配方,增殖系数可达7.3;将3 cm长的嫩茎经壮苗培养和MS+0.25 mg/L BA处理后,转接到1/2MS+0.25 mg/L IBA+0.1 mg/L NAA+0.2 g/L活性炭,且pH为6.5生根培养基中,生根率高达87%以上。在增殖培养基中添加1.0 g/L的50%多菌灵可湿性粉剂能有效地抑制内生菌。
【Abstract】 Tissue culture technics of Nandina domestica was studied in this paper.The results showed that the most appropriate proliferation formula was MS+1.5 mg/L BA+0.1 mg/L IBA,its proliferation coefficient was 7.3.When young stems which were cultured in a medium(MS+0.25 mg/L BA) reached 3 cm long,They were transferred into a rooting culture medium [1/2MS+0.25 mg/L IBA+0.1 mg/L NAA+0.2 g/L AC(pH=6.5)]. The rooting rate could reach over 87%.The proliferation medium with 1.0 g/L 50% carbendazol wettable powder added could effectively inhibit endophyte in plants.
- 【文献出处】 林业科技开发 ,China Forestry Science and Technology , 编辑部邮箱 ,2011年02期
- 【分类号】S687
- 【被引频次】8
- 【下载频次】467