【摘要】 提出了高效液相色谱-质谱法测定青藏高原植物和土壤样品中氨基酸的含量。样品中氨基酸经6mol·L^-1盐酸溶液提取,加入衍生试剂1,2-苯并-3,4-二氢咔唑-9-乙基氯甲酸酯（BCEOC）进行衍生化反应,衍生化产物经Hypersil BDS C₁₈（4.6mm×200mm,5μm）色谱柱分离,于激发波长(λ_ex)333 nm和发射波长(λ_em)390nm进行荧光检测定量,用质谱法定性。色谱分离中采用不同体积比的乙腈与水的混合溶液梯度洗脱,在质谱检测中采用电喷雾离子源正离子模式。方法的检出限（3S/N）为6.3×10^-15～1.8×10^-13mol之间。以土壤样品为基体,加入20种氨基酸标准作回收试验,测得回收率在89.0%～107.0%之间。更多还原

【Abstract】 HPLC-MS was applied to the determination of amino acids in plants and soil of Qing-Zang plateau. The amino acids were extracted from the sample with 6 mol·L^-1 HCl solution and derivatized with 1,2-benzo-3,4- dihydrocarbazole-9-ethyl chloroformate（BCEOC）.The derivatized products were separated by gradient elution with mixed solution of acetonitrile and water of different ratios on Hypersil HDS C₁₈ column（4.6 mm×200 mm,5μm）. The amino acids were determined by fluorescence detector at the wavelength of 333 nm(λ_ex) and 390(λ_em) and identified by MS with ESI⁺.Detection limits（3S/N） found were in the range of 6.3×10^-15 - 1.8×10^-13 mol. Standards of 20 amino acids were added to extracts of soil sample and tested for the recovery;values of recovery obtained were in the range from 89.0%to 107.0%.更多还原