【摘要】 [目的]探讨葡萄籽油（grape seedoil,GSO）对氯化镉（CdCl₂）诱导大鼠肝脏细胞DNA损伤的保护作用。[方法]将40只SPF级雌雄各半SD大鼠随机分为对照组（腹腔注射生理盐水和灌胃生理盐水）、染镉组（腹腔注射1.0mg/kg的CdCl₂和灌胃生理盐水）、GSO低、中、高剂量干预组（均腹腔注射1.0mg/kg CdCl₂,同时分别灌胃0.66、1.32、2.64mg/kgGSO）,硫代巴比妥酸法检测肝中丙二醛（malondialdehyde,MDA）的含量,采用单细胞凝胶电泳技术（SCGE）检测各组肝脏细胞DNA损伤情况。[结果]1.0mg/kgCdCl₂可诱导大鼠肝脏细胞合成MDA,使其含量明显增加并加速DNA的氧化损伤;GSO高剂量干预组中MDA含量较染镉组差异具有统计学意义（P<0.05）,3个GSO干预组MDA的清除率分别为54.55%、54.55%和100.00%,不同剂量的GSO对MDA清除呈较好的剂量-效应关系。各GSO干预组DNA氧化损伤较染镉组均明显下降（P<0.05）。[结论]一定剂量葡萄籽油可降低大鼠肝脏中MDA含量和镉致DNA氧化损伤,具有明显的抗脂质过氧化损伤的作用。更多还原

【Abstract】 [Objective]To explore the protective effect of grape seed oil（GSO） on DNA oxidative damage of liver cells in rats exposed to CdCl₂.[Methods]A total of 40 SD rats of SPF grade were randomly divided into 5 groups,which were the control group（intraperitoneal injection and gastric perfusion of normal saline with same volume）,the CdCl₂ exposure-positive group （intraperitoneal injection of 1.0mg/kg CdCl₂ and gastric perfusion of normal saline with the same volume）,and the GSO low-,medium- and high-dose intervention groups（intraperitoneal injection of 1.0mg/kg CdCl₂ and gastric perfusion of 0.66mg/kg,1.32mg/kg,2.64mg/kg GSO,respectively） with 8 of either gender in each group.Malondialdehyde（MDA） content in liver was measured by thiobarbituric acid（TBA） method.DNA oxidative damage was measured by single cell gel eletrophoresis（SCGE）.[Results]The 1.0mg/kg CdCl₂ induced a significant increase in MDA content and DNA oxidative damage.The difference of MDA contents between the GSO high-dose intervention group and the CdCl₂ exposure-positive group was significant（P<0.05）.The clearances in the GSO low-,medium- and high-dose intervention groups were 54.55%,54.55%and 100.00%,respectively.The DNA damage significantly decreased in the GSO intervention groups in a dose-dependent manner,compared with the CdCl₂ exposure-positive group（P<0.05）. [Conclusion]A certain dose of grape seed oil can induce a decrease in MDA content and DNA damage in rat liver after cadmium exposure.更多还原