【摘要】 目的建立一种柱前衍生-高效液相色谱法测定肠内营养剂中脯氨酸的含量。方法以氯甲酸-9-芴基甲酯（FMOC-Cl）为衍生化试剂,色谱柱采用Agilent C₁₈（150 mm×4.6 mm,5.0μm）柱,流动相为乙腈-50 mmol·L^-1醋酸钠缓冲液（pH4.2）,梯度洗脱,流速为1.0 mL·min^-1,柱温30℃;FLD荧光检测,激发波长263 nm,发射波长315nm。待测肠内营养剂样品经10 mol·L^-1盐酸溶液溶解稀释后,超声提取,在室温条件下与衍生化试剂反应15 min,反应终止并取20μL进样分析。结果脯氨酸在质量浓度0.1～1 mg·L^-1内线性关系良好,r=0.999 6;平均回收率109.42%～112.72%,日间、日内精密度RSD均<7%;样品溶液在24 h内稳定。结论该方法灵敏、简便、专属性强,能对肠内营养剂中的脯氨酸进行准确的定量分析,并能避免制剂中其他氨基酸组分的干扰。更多还原

【Abstract】 AIM To develop a pre-column derivatization-HPLC method for the determination of proline in enteral nutrition.METHODS An Agilent C₁₈ column（150 mm×4.6 mm,5.0μm） was used with a mobile phase consisting of acetonitrile-sodium acetate(pH 4.2,50 mmol·L^-1) for gradient elution analysis.The flow rate was 1.0 mL·min^-1. The column temperature was 30℃.Theλ_ex of FLD was 263 nm and theλ_em was 315 nm.The derivatization reagent was 9-fluorenylmethyl chloroformate（FMOC-Cl）.RESULTS The linear range of proline was 0.1-1 mg·L^-1（r= 0.999 6）.The range of average recoveries was 109.42%-112.72%.Both the inter-day RSD and the intra-day RSD were less than 7%.The sample solvent was stable in 24 h.CONCLUSION The method is simple,sensitive and specific which can be applied for determination of proline in enteral nutrition and for quality control of the preparation.更多还原