【摘要】 目的:探讨人胰腺癌肿瘤相关钙信号传导蛋白2（tumo-associated calcium signal transducer-2,TROP-2）基因在胰腺癌组织中表达及其临床意义。方法:提取56例人胰腺癌及癌旁正常组织中总mRNA,采用实时荧光定量逆转录聚酶链反应（RT-PCR）法检测其中TROP-2 mRNA表达情况;并分析其表达与患者年龄、肿瘤大小、淋巴结转移、肿瘤分化程度和TNM分期相关性。结果:以2^-ΔΔCt≥2为阳性标准,TROP-2在胰腺癌组织中阳性表达率为75.0%（42/56）,而癌旁正常组织未见TROP-2 mRNA表达,两者差异有统计学意义（P<0.001）。TROP 2 mRNA过表达与肿瘤大小、淋巴结转移、TNM分期显著相关（均P<0.01）,而与患者年龄和肿瘤分化程度无关（均P>0.05）。结论:TROP-2基因表达与胰腺癌进展密切相关,可能是反映胰腺癌侵袭、转移的重要指标之一。更多还原

【Abstract】 Objective:To investigate the expression and significance of tumor-associated calcium signal transducer-2（TROP-2） in human pancreatic cancer tissue.Methods:The expression of TROP-2 mRNA in 56 samples of pancreatic cancer tissues and adjacent normal tissues were determined by real time RT-PCR,and 2^-△△Ct≥2 as a positive standard.Results:The positive rate of TROP-2 expression in pancreatic cancer tissue was 75.0%,however,tumor adjacent tissue was 0%.In addition,TROP-2 over-expression was related with lymph node metastasis and TNM stage,but not with age and tumor pathological grading.Conclusions: TROP-2 is closely correlated to pancreatic cancer progression,and TROP-2 might be a potential marker for metastasis of pancreatic cancer.更多还原