【摘要】 目的:检测原发性高血压(EH)伴颈动脉粥样硬化患者外周血Th17细胞比例的变化及与血管紧张素Ⅱ(AngⅡ)水平的相关性。方法:行颈动脉超声检查选择20例EH伴颈动脉粥样硬化患者(CA组),颈动脉正常的25例EH患者(EH组)以及20例性别、年龄匹配的健康体检者作为对照组。以流式细胞术检测外周血中Th17细胞比例,ELISA法检测血浆中IL-17水平,放射免疫法检测血浆AngⅡ水平。结果:与EH组和对照组相比较,CA组Th17细胞比例显著升高,血浆IL-17水平显著升高。CA组AngⅡ水平高于EH组和对照组,EH组与对照组间AngⅡ水平差异无统计学意义。AngⅡ水平与Th17细胞比例、血浆IL-17水平呈正相关。结论:EH患者的AngⅡ水平和Th17细胞活性升高,这种改变可能与颈动脉粥样硬化的发生发展有关。更多还原

【Abstract】 Objective:The goal of the present study was to elucidate the relationship between angiotensin Ⅱ level and Th17 cells in hypertensive patients with carotid atherosclerosis.Method:Forty-five hypertensive patients were divided into two groups by B-mode ultrasound inspection: group one consisting of 20 cases with carotid atherosclerosis(CA),and group two consisting of 25 cases without carotid atherosclerosis(EH).The control group consists of 20 health subjects without carotid atherosclerosis.Peripheral blood mononuclear cells were collected in a fasting state.The frequencies of Th17 cells were analysed by flow cytometry.The levels of plasma angiotensin Ⅱ were measured by radioimmunoassay and the levels of IL-17 were measured by ELISA.Result:Compared with that in EH and control group,the frequencies of Th17 cells in CA group were significantly increased,companied with a higher level of IL-17.Furthermore,the levels of angiotensin Ⅱ in CA group were significantly higher than that in EH and control group.Conclusion:Rescults collectively suggest that the changes of angitension Ⅱ and Th17 cells in hypertensive patients propably contribute to the development of carotid atherosclerosis.更多还原