【摘要】 目的研究腺苷A1受体激动药2-氯环戊腺苷(CCPA)预处理延迟相对缺血-再灌注损伤心肌αB-晶状体蛋白的影响。方法新西兰大白兔12只,随机均分为三组:假手术组(S组)、生理盐水预处理组(N组)、CCPA预处理组(C组)。S组分离兔左冠状动脉前降支,套带,但不阻断血流;N、C组在用药24h后分离兔左冠状动脉前降支,结扎30min,恢复灌注2h。提取心肌组织蛋白,采用WesternBlotting检测αB-晶状体蛋白的表达。结果 S组中αB-晶状体蛋白表达很少,C组αB-晶状体蛋白表达显著高于S、N组(P<0.01)。结论 CCPA预处理延迟性心肌保护作用与αB-晶状体蛋白表达上调有关。更多还原

【Abstract】 Objective To investigate the effects of delayed preconditioning mediated by 2-chloro-N6-cyclopentyl-adenosine(CCPA)on myocardial αB crystallin.Methods Twelve rabbits were randomly divided into three groups:group S,group N and group C.CCPA was infused into rabbit in group C,while the same volume of normal saline(NS)was given instead in group N on day 1,no treatment was done in group S.24 hours after CCPA or NS pretreatment,rabbits in groups C and N were subjected to 30 min left anterior descending coronary artery(LAD)occlusion and reperfused for 2 h.Rabbits in group S did not subjecte to LAD occulsion and reperfusion.Myocardial αB crystallin was determinned with western blotting.Results αB crystallin was up-regulated by the CCPA pretreatment.Conclusion Delayed myocardial protection by CCPA pretreatment was relevant with up-expression of myocardial αB crystallin.更多还原