【摘要】 目的探讨睡眠剥夺(sleep deprivation,SD)对动脉粥样硬化(atherosclerosis,AS)进程的影响及其机制。方法 50只Wistar AS雄性大鼠随机分为5组(每组10只):AS组、SD1d组、SD3d组、SD5d组、SD7d组。建立AS大鼠SD模型,制备主动脉组织标本,苏木精伊红(hematoxylin and eosin,HE)染色,光镜观察切片。检测主动脉组织还原型谷胱甘肽(glutathione,GSH)及脂质过氧化物丙二醛(malondialdehyde,MDA)含量、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)及超氧化物歧化酶(superoxide dismutase,SOD)活性。检测血清肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、高敏C反应蛋白(hypersensitivity-C reactive protein,hsCRP)、白细胞介素6(interleukin-6,IL-6)水平。结果随着睡眠剥夺时间的延长,大鼠AS病变进展逐渐加重。主动脉组织GSH含量逐渐降低,MDA含量逐渐增高,GSH-Px及SOD活性逐渐降低。血清中TNF-α、hsCRPI、L-6水平也在逐渐增高。结论睡眠剥夺可通过引起机体的氧化应激反应及加速炎症发展,从而促进AS进程。更多还原

【Abstract】 Objective To study the effects of sleep deprivation on the atherosclerotic process and its mechanism in rats.Methods Fifty rats were randomly divided into five groups(10 each): AS group,SD1d group,SD3d group,SD5d group and SD7d group.After five groups were established AS model,four groups were established sleep deprivation model,the aortic tissue was prepared,stained with hematoxylin and eosin,and then observed by light microscopy.The contents of GSH,MDA and the activity of GSH-Px,SOD in aortic tissue were detected.The levels of TNF-α hsCRP and IL-6 in serum were detected.Results With the time of sleep deprivation in rats,the lesions progressively became worsened.As the content of MDA was gradually increased,GSH content,GSH-Px and SOD activity were gradually decreased,serum TNF-α,hsCRP,IL-6 activity were also increased gradually.Conclusion Sleep deprivation can cause oxidative stress in the body and accelerate the development of inflammation,thus contributing to the process of atherosclerosis.更多还原