【摘要】 目的:探讨胰岛素样生长因子-1受体(IGF-ⅠR)在鼻息肉组织中的表达及其与变应性因素的关系。方法:使用荧光定量PCR技术和免疫组织化学技术分别检测40例伴鼻息肉的慢性鼻-鼻窦炎患者的鼻息肉组织(CRSWNP组)和20例鼻中隔偏曲患者的中鼻甲组织(对照组)中IGF-ⅠR mRNA及其蛋白的表达并进行统计学分析。结果:IGF-ⅠR蛋白在CRSWNP组及对照组的阳性率分别为70.8%和12.3%,IGF-ⅠR mRNA在2组的表达分别为0.748±0.111和0.107±0.208,CRSWNP组IGF-ⅠR蛋白和IGF-ⅠR mRNA的表达均显著高于对照组(均P<0.01);CRSWNP组中,有、无鼻内镜手术史和有、无合并变应性因素组间的IGF-ⅠR mRNA表达均差异无统计学意义(均P>0.05)。结论:IGF-ⅠR在CRSWNP的发病机制中发挥作用,变应性因素不是IGF-ⅠR的表达的直接影响因素。更多还原

【Abstract】 Objective:To study the expression of insulin-like growth factor-1 receptor(IGF-ⅠR) in nasal polyps and its relationship with allergic rhinitis.Method:The mRNA and protein expression of IGF-ⅠR in 40 cases(20 cases with allergic rhinitis and 20 cases without) nasal polyps tissue(the CRSWNP group) and 20 middle turbinate tissue samples(the control group) were examined by fluorescence quantitative polymerase chain reaction(FQ-PCR) and immunohistochemistry.Result:The positive stainning rate of IGF-ⅠR protein of nasal polyps tissue is 70.8% and that of control is 12.3%,the expression of IGF-ⅠR mRNA of nasal polyp is 0.748±0.111,which is significant higher than that of the control group is 0.107±0.208(P<0.01).NO significant difference of the expression of IGF-ⅠR mRNA between with and without allergic rhinitis cases and between with and without endoscopy sinus surgery history cases in the CRSWNP group.Conclusion:The overexpression of IGF-ⅠR maybeplay important roles in the formation of nasal polyp.Hypersensitivity reaction type I mediated by IgE has no contribution to the overexpression of the IGF-ⅠR.更多还原