【摘要】 目的建立肥胖大鼠模型,观察肥胖大鼠血清脂联素的变化及动脉血管内皮核因子(NF)-κB、血管内皮细胞黏附分子(VCAM)-1、细胞间黏附分子(ICAM)-1的表达,探讨其在血管病变发生发展中的作用机制。方法 4周龄雄性健康SD大鼠48只,随机分为对照组(18只)和高脂组(30只),给予高脂饲料制备肥胖大鼠模型。8周后,以高脂组大鼠的体质量超过对照组大鼠平均体质量的20%作为肥胖组大鼠。用酶联免疫法检测血清脂联素含量,应用免疫组化技术测定NF-κB、VCAM-1、ICAM-1在血管内皮的表达。结果高脂组大鼠平均体质量明显高于对照组,(446.82±10.05)g比(365.21±13.20)g,P<0.01;高脂组中有22只大鼠体质量超过对照组平均体质量20%,作为实验用肥胖大鼠(肥胖组)。肥胖组大鼠血清脂联素水平明显低于对照组,(0.61±0.13)ng/ml比(1.00±0.17)ng/ml,P<0.01,动脉血管内皮NF-κB、VCAM-1、ICAM-1阳性表达显著高于对照组,且肥胖组大鼠血清脂联素水平与血管内皮NF-κB、VCAM-1、ICAM-1表达均呈明显负相关(r=-0.938～-0.891,P均<0.001)。结论高脂饮食引起的肥胖可使血清脂联素水平降低,使脂联素对NF-κB的抑制减弱,导致肥胖大鼠血管内皮VCAM-1、ICAM-1表达的增加,从而使动脉血管内膜增厚,血管结构发生改变导致血管疾病的发生。更多还原

【Abstract】 Objective To establish an obese rat model,to observe the level of serum adiponectin and the expression of NF-κB,VCAM-1,ICAM-1,to explore the mechanism of angiopathy.Methods Forty-eight four-week-old healthy male SD rats were randomly divided into control group(n = 18)and high-fat group(n = 30).The high-fat group was fed the high-fat diet to establish the obese rat model.After 8 weeks,the rats in high-fat group with body weight more than 20% of average body weight of control group were selected as the obese rats.The level of serum adiponectin was measured by Enzyme-Linked Immuno Sorbent Assay.The expression of NF-κB,VCAM-1 and ICAM-1 in vascular endothelium was detected by immunohistochemistry.Results The average body weight was significantly heavier in high-fat group than in control group(446.82 ± 10.05 g vs.365.21 ± 13.20 g,P < 0.01).Twenty-two rats were selected as obese rats in high-fat group(obese group),whose body weight was 20% more than the average weight of control group.The level of serum adiponectin were significantly lower in obese rats than in control group(0.61 ± 0.13 ng/ml vs.1.00 ± 0.17 ng/ml,P < 0.01).The expression of NF-κB,VCAM-1,ICAM-1 in vascular endothelial was significantly higher in obese group than in control group.Meanwhile,the adiponectin levels were negatively correlated with NF-κB,VCAM-1,ICAM-1 in obese rats(r =-0.938 to-0.891,P < 0.001).Conclusion In obesity due to high-fat diet the level of serum adiponectin may be reduced and the inhibition of the serum adiponectin on NF-κB may be decreased,and thus the expression of VCAM-1 and ICAM-1may be increased.It causes the accumulation of artery vessel thickening and change,and causes the vascular disease.更多还原