【摘要】 本文以微藻(莱茵衣藻)为材料,通过室内模拟石灰岩土壤系统,对接种微藻的土柱进行不同淋滤条件的处理,系统监测了淋出液的电导率、Ca2+浓度和碳酸酐酶的活性。结果表明,以双蒸水进行淋滤的土柱,Ca2+总淋出量在24 d时仅为以微藻培养液进行淋滤土柱的11.9%,且淋出液碳酸酐酶的平均活性与Ca2+总淋出量间有较好的相关性,说明微藻及其碳酸酐酶对石灰岩土壤系统中的Ca元素迁移有较强的驱动作用。本文的研究结果为探讨藻类碳酸酐酶在岩溶发育中的作用提供了一定的科学依据,进一步深入研究将有助于弄清藻类及其碳酸酐酶对岩溶碳汇的作用。更多还原

【Abstract】 Chlamynonas reinhardtii was selected as the target microalgae and was inoculated onto simulative soil-limestone columns.This study systematically measured dynamics of electric conductivity,Ca2+ concentration and carbonic anhydrase activity in leachates under different leaching conditions.The results showed that,when the columns were leached with redistilled water,the total amount of leached Ca2+ at the time of 24 d was 11.9% of that leached with culture liquid of microalgae,and that the average activity of carbonic anhydrase in the leachates was correlated well with the total amount of leached Ca2+.These results implied that microalgae and its carbonic anhydrase have significant driving effect on calcium migration in the soil-limestone karst system.The results of this paper provide scientific basis for studying the role of algae carbonic anhydrase in karst development.It is expected that a deepen investigation following this study will help in clarifying the role of algae and the carbonic anhydrase in carbon sequestration of karst areas.更多还原