【摘要】 目的探讨VDR基因多态性与云南汉族散发性乳腺癌的关联.方法用聚合酶链反应-限制性长度多态性(PCR-RFLP)试验方法,对80例乳腺癌患者和80例正常人的VDR的TaqI多态位点进行检测,确定出VDR三种基因型,即TT、Tt、tt,用卡方、秩和检验对实验组和对照组进行统计分析.结果 VDR基因型TT、Tt和tt三种基因型的分布频率,乳腺癌患者组中分别是36.3%、48.6%、15.0%,对照组中分别是38.6%、46.3%和15.0%;乳腺癌患者组中T、t等位基因频率分别为60.6%、39.4%,对照组为61.8%、38.2%,VDR基因型及等位基因在两组间分布频率的差异无统计学意义(P>0.05).结论 VDR基因中Taq I酶切位点导致的基因多态性与云南散发性乳腺癌无关联,但由于VDR基因的酶切位点较多,具体了解此基因多态性与乳腺癌易感性的关联,需要对BsmI、Apa I、Fok I等酶切位点进一步实验,才能对其有一个更客观的认识.更多还原

【Abstract】 Objective To investigate the relationship between genetic polymorphisms of Vitamin D receptor and the susceptibility of breast cancer in Yunnan women of han nationality.Methods VDR gene polymorphisms were detected using polymerase?chain reaction-restriction fragment length polymorphism(PCR-RFLP)analysis,and three genotypes were identified.VDR gene polymorphisms were obtained from a Yunnan population-based case-control study including 80 sporadic breast cancer and 80 normal controls.Chi-square test and rank test were used to analyze the data.Results The VDR genotypes identified were TT,Tt and tt.In breast cancer group,the prevalance rate of VDR geneotype TT,Tt and tt was 36.3%,48.6% and 15.0%,respectively.In the control group,the prevalance rate of VDR geneotype TT,Tt and tt was 38.6%,46.3% and 15.0%,respectively.The frequencies of VDR T and t alleles were 60.6% and 39.4%,respectively,in breast cancer group and 61.8% and 38.2%,respectively,in the control group.There was no significant difference in VDR genotype and allele frequencies between the two groups(P>0.05).Conclusions The genetic polymorphisms of Vitamin D receptor caused by Taq I restriction enzyme cutting site are not related with更多还原