【摘要】 目的研究高血脂与动脉粥样硬化基因的相关性,从中筛选动脉粥样硬化早期诊断基因。方法采用温和的动脉粥样硬化膳食(0.22 mg胆固醇/卡路里,40%的能量来源于脂肪)单笼喂养中老年雄性食蟹猴,膳食诱导12个月后,从膳食诱导实验猴中筛选出10只高血脂猴。然后采用荧光定量PCR方法检测对照组和高血脂组食蟹猴外周血白细胞内113个动脉粥样硬化相关基因的表达差异。结果在食蟹猴外周血白细胞内共检测到66个动脉粥样硬化相关基因,其中18个基因随血脂升高表达显著上调(P<0.05),21个基因表达显著下调(P<0.05),另外还有27个基因无表达差异(P>0.05)。结论以上结果为后续大样本相关研究缩小了基因遴选范围,通过进一步研究可能从中筛选出基因表达水平上的动脉粥样硬化早期诊断指标。更多还原

【Abstract】 Aim Researching the correlation of atherosclerosis related genes and hyperlipidemia,in order to find atherosclerosis early diagnosis target.Methods Middle-aged male crab-eating Macaque(Macaca fascicularis) were fed moderately atherogenic diet(0.22 mg cholesterol/kcal and 40% of calories as saturated fat) for 12 months.According to blood-lipid hyperlipidemia crab-eating Macaques(n=10) were selected,then the expression differences of 113 atherosclerosis related genes were analysed by real-time PCR.Results 66 genes were detected in peripheral blood leucocyte.There were 18 genes upregulation and 21 genes downregulation with elevated blood lipid(P<0.05),besides 27 irregular genes.Conclusions Above result narrowed the scope of genes selection for further research,through further research we may select atherosclerosis early diagnosis target.更多还原