【摘要】 目的探讨益赛普对大鼠角膜新生血管形成和血管内皮生长因子(VEGF)表达的影响。方法健康Wister大鼠45只,随机分为3组:正常组、生理盐水对照组、益赛普治疗组,建立角膜缝线诱导的大鼠角膜新生血管的动物模型,治疗组和对照组分别给予0.1 mg/0.1 mL益赛普和0.1 mL生理盐水结膜下注射,采用裂隙灯观察角膜新生血管的生长情况,用免疫组织化学方法检测VEGF的表达。结果各时间点新生血管的面积,治疗组低于对照组(P<0.05)。免疫组化显示,7、10、14 d治疗组VEGF的表达较对照组明显减少(P<0.05)。结论益赛普通过下调VEGF的表达,可有效抑制角膜缝线诱导的大鼠角膜新生血管形成。更多还原

【Abstract】 Objective To investigate the effect on the expression of vascular endothelial growth factor(VEGF) in corneal neovascularization(CRNV) of rats,study the functional mechanism of Etanercept in inhibiting CRNV and offer the new theory for inhibiting CRNV.Methods Nylon suture induced corneal neovascularization.CRNV model rats were randomly divided into three groups: normal group,the experimental group(received 0.1 mg/0.1 mL etanercept) and the control group(treated with physiologic saline).The growth of corneal neovascularization was observed under slit lamp microscopy.The expression of VEGF in CRNV of each rat was detected by immunohistochemical staining.Results Compared with the control group,the growth of CRNV in experimental group at various time points reduced significantly(P<0.05).Immunohistochemistry showed that VEGF in experimental group was lower than that in control group.Conclusion Etanercept can efficiently inhibit the growth of CRNV induced by cornea seam through reducing the expression of VEGF in cornea.更多还原