【摘要】 目的分析铜绿假单胞菌的耐药性及其耐药基因类型,指导临床合理应用抗生素。方法收集广州中医药大学第一附属医院临床送检标本中分离的50株铜绿假单胞菌,用K-B纸片扩散法进行抗生素敏感性试验;筛选出20株多重耐药铜绿假单胞菌,采用聚合酶链反应检测β-内酰胺酶基因类型。结果铜绿假单胞菌对临床常用抗生素存在不同程度的耐药现象,其中最常用的亚胺培南耐药率达96%,20株多重耐药铜绿假单胞菌中检出TEM(40%)、OXA-2群(5%)、OXA-10群(5%)、CARB(30%)4种β-内酰胺酶基因,未检出质粒型AmpC酶和金属β-内酰胺酶基因。膜孔蛋白编码基因oprD2均为缺失型。结论铜绿假单胞菌的耐药现象严重,其原因可能与铜绿假单胞菌多耐药基因表达有关。更多还原

【Abstract】 Objective To analyze the drug resistance and the genes types of Pseudomonas aeruginosa,and to provide guidelines for the reasonable application of clinical antibiotic.Methods 50 strains of Pseudomonas aeruginosa were collected from clinical inspection specimens,the antimicrobial drug sensitivity of which were tested by K-B disc diffusion method.20 strains of multiple drug-resistant Pseudomonas aeruginosa were screened,and we used polymerase chain reaction(PCR) method to detect beta-lactamase gene types.Results The Pseudomonas aeruginosa had various degrees of drug resistance.The irologic resistance which was the most commonly used imipenem reached 96%,followed by four genes of beta-lactamase,namely TEM(40%),OXA-2 group(5%),OXA-10 group(5%),and CARB(30%),but plasmid model AmpC enzyme and metal beta-lactamase genes were not detected,and the film hole protein coding genetic oprD2 were missing from all 20 strains of pseudomonas aeruginosa of multiple drug-resistant.Conclusion Drug-resistance of Pseudomonas aeruginosa is serious,the cause of which might be related with the resistant gene that Pseudomonas aeruginosa expresses.更多还原