【摘要】 目的为了解儿童血清透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PcⅢ)、Ⅳ型胶原(CⅣ)水平以及在慢性肝炎中的临床价值。方法采用酶联免疫吸附试验测定400例1~15岁健康儿童和180例患有慢性肝炎儿童的4项肝纤维化标志物。结果 4项标志物检测结果(x±s)如下:HA(58±16)ng/mL,LN(115±17)ng/mL,PcⅢ(146±27)ng/mL,CⅣ(51±16)ng/mL。HA、CⅣ与年龄不相关(r=-0.022,P>0.05;r=0.070,P>0.05),而LN与年龄显著相关(r=0.128,P<0.01),PcⅢ与年龄呈负相关(r=-0.669,P<0.01)。慢性乙型肝炎表面抗原携带者与同龄对照组差异无统计学意义。轻度慢性肝炎患儿除HA显著高于对照组,差异有统计学意义(P<0.05)外,其他3项差异均无统计学意义。中度、重度及肝硬化儿童的4种血清标志物浓度均高于健康对照组(P<0.01)。在中度、重度及肝硬化儿童中,有两种或3种以上的标志物显著性增加。结论 4项标志物在儿童期有其年龄特征。它们可反映慢性肝炎患儿肝纤维化的严重程度。更多还原

【Abstract】 Objective To investigate the clinical value of serum hyaluronic acid(HA),laminin(LN),type Ⅲ prcollagen(PcⅢ)and type Ⅳ collagen(CⅣ) levels in children with chronic hepatitis.Methods The 4 serum markers of liver fibrosis in 400 normal children and 180 children with chronic hepatitis aged 1~15 years old were determined by ELISA.Results The results of determination of the 4 markers were as follows(x±s):HA(58±16)ng/mL,LN(115±17)ng/mL,PcⅢ(146±27)ng/mL,CⅣ(51±16)ng/mL.NO correlation was found between age and HA,CⅣ(r=-0.022,P>0.05and r=0.070,P>0.05),but there was a positive correlation between LN and age(r=0.128,P<0.01),and there was a negative correlation between PCⅢ and age(r=-0.669,P<0.01).The difference between the values of chronic HBsAg carriers and those of the normal controls at the same age was not significant.The values of the mild chronic hepatitis patients were not significantly different from those of the controls except for HA,which was higher than that of the controls(P<0.05).The serum concentrations of all these markers in children with moderate,severe chronic hepatitis and cirrhosis were significantly increased compared with those of the controls(P<0.01).2 or more markers increased was seen in patients with moderated and severe chronic hepatitis and liver cirrhosis.Conclusion The 4 serum markers had certain characteristics with regard to the of children,Which may reflect the degree of liver fibrosis in children with chronic hepatitis.更多还原