【摘要】 目的探讨不同真空采血管对高效液相色谱法检测全血糖化血红蛋白(HbAlc)的影响。方法利用4种真空采血管分别采集40例门诊患者静脉血,在RIO-RAD VariantⅡ型糖化血红蛋白分析仪上测定结果,并对样本进行重复冻融试验。结果紫色帽(K2-EDTA)7.88%±1.2%、灰色帽(氟化钠/肝素钠)7.96%±1.2%、绿色帽(肝素锂)7.91%±1.2%、蓝色帽(柠檬酸钠)7.95%±1.2%。不同抗凝剂真空采血管采集的HbAlc样本结果差异无统计学意义(P>0.05)。重复冻融3次后紫、灰、绿帽组结果与原始结果比较差异有统计学意义(P<0.05)。结论四种真空采血管均可用来采集全血糖化血红蛋白样本,样本重复冻融3次以上对结果有影响。更多还原

【Abstract】 Objective To study the effect of different vacuum blood sampling tubes on whole blood glycohemoglobin(HbAlc) detected by high performance liquid chromatography(HPLC).Methods Venous blood samples were collected from 40 outpatients with 4 kinds of vacuum blood sampling tubes.The samples were frozen and then thawed for measuring HbA1c with the RIO-RAD VariantⅡHPLC analyzer.Results The HbA1c was 7.88%±1.2%,7.96%±1.2%,7.91%±1.2%,and 7.95%±1.2% in samples collected with purple hat(K2-EDTA),gray hat(sodium fluoride/heparin sodium),green hat(heparin lithium) and blue hat(sodium citrate) respectively,which was not significantly different from those collected with vacuum blood sampling tubes(P>0.05).After 3 times of freezing and thawing,the HbA1c in samples collected with purple,gray and green hats was significantly different from those collected with different blood sampling tubes(P<0.05).Conclusion Vacuum blood sampling tubes can be in collecting whole blood HbA1c samples.Over three times of freezing and thawing of the sample can influence the detection of HbA1c.更多还原