【摘要】 目的探讨哮喘患儿诱导痰中TGF-β1表达、IL-5水平及E0S计数与肺功能的相关性。方法选取30例5-15岁支气管哮喘急性发作患儿(哮喘组)、该组30例经吸入糖皮质激素治疗6个月-1年进入哮喘缓解期的患儿(缓解组),30例5-15岁健康儿童(对照组),对3组病人测定诱导痰中TGF-β1表达、IL-5水平及E0S计数,3组病人均测定肺功能FEV1等值。结果(1)哮喘组TGF-β1阳性细胞表达率与缓解组比较,P<0.01;哮喘组IL-5、E0S与对照组、缓解组比较,P均<0.01;(2)哮喘组FEV1%pre、FEF25-75%均低于对照组及缓解组,P<0.05;缓解组FEV1%pre与对照组比较,差异无统计学意义;缓解组FEF25-75%低于对照组,P<0.05;(3)哮喘组痰液中TGF-β1阳性细胞表达率与IL-5水平、EOS%呈正相关:r=0.38 P<0.05,r=0.66,P<0.01;与肺功能FEV1%pre、FEF25-50呈负相关:r=-0.68,P<0.01,r=-0.555,P<0.05。结论 TGF-β1、IL-5、EOS在哮喘患儿诱导痰中有过度表达,这些指标与肺功能有很好的相关性,可作为气道慢性炎症的一个标示物用来监测哮喘。更多还原

【Abstract】 Objective To study the correlation between expressing of TGF-β1,IL-5 level,EOS count in induced sputum and pulmonary function of children with actue asthma.Methods Selection of 30 cases of 5 to 15 years old the asthma patients(asthma group),they inhaled corticosteroids for six months-1 years into remission of asthma patients(ease group),30 cases of 5 to 15 years old healthy children were served as the control group,Expressing of TGF-β1,the IL-5 level,EOS count in induced sputum was determined,The forced expiratory ratio(FEV1) were measured on the three groups of patients.Results(1)The positive expression of TGF-β1 in inflammatory cells in induced sputum were higher in asthma group than in ease group P﹤0.01;The expression of IL-5 and EOS count were higher in asthma group than in control group and ease group,P<0.01 respectively.(2)Asthma group FEV1% pre,FEF25-75% were lower than those of the control group and ease group,P<0.05 respectively;the difference between FEV1%pre of control ease group and control group were no statistically significant;The FEF25%-75% were lower in ease group than in control group,P<0.05.(3)The positive expression of TGF-β1 in inflammatory cells in induced sputum of asthma group were positively correlated with IL-5 level and EOS count r=0.38,P<0.05,r=0.66,P<0.01,and negative correlation with FEV1%pre,FEF25-50%,r=-0.68,P<0.01,r=-0.555,P<0.05.Conclusion The over-expression of TGF-β1,IL-5,EOS count in induced sputum of asthma patients,have a good correlation with pulmonary function,can be used as a chronic inflammatory airway markers to monitor asthma.更多还原