【摘要】 目的建立一种同时测定大鼠蛇床子素和欧前胡素的血药浓度的方法。方法采用迪马Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为:甲醇∶水=70∶30(v/v),流速为1.0 mL.min-1,紫外检测波长为320 nm,米非司酮为内标。取大鼠血样90μL进行血浆样品的处理,测定大鼠血浆中蛇床子素和欧前胡素的色谱行为、标准曲线、提取回收率、精密度和准确度、稳定性等。结果蛇床子素、欧前胡素在大鼠血浆中质量浓度测定方法的线性范围分别为10.94～700.00μg.L-1和15.63～1 000.00μg.L-1,线性关系均良好(r=0.999 8、0.999 9,均P<0.05)。大鼠血浆蛇床子素、欧前胡素最低可定量的质量浓度分别为10.94、15.63μg.L-1(以s/n>4计)。大鼠血浆蛇床子素、欧前胡素低、中、高质量浓度的提取回收率分别为98.96%、95.30%、94.88%和98.23%、98.94%、97.99%,大鼠血浆蛇床子素和欧前胡素低、中、高质量浓度的RSD均<15%。结论建立HPLC法具有检测简便、灵敏度高等优点,可快速、可靠地检测大鼠蛇床子素和欧前胡素的血药浓度。更多还原

【Abstract】 Objective To establish a method for simultaneous detection of osthol and imperatorin in rat blood drug level concentrations.Methods HPLC was performed on Dima Diamonsil C18 reversed-phase column(250 mm×4.6 mm,5 μm) with mobile phase of methanol-water(70∶30,v/v).The eluate was monitored by UV absorption at 320 nm.The flow rate was 1.0 mL·min-1.Mifepristone was served as an internal standard.Plasma samples from 90 μL blood were prepared,and chromatographic behavior,standard curve,extraction recovery rate,precision,accuracy and stability of osthol and imperatorin were analyzed.Results The linear range of the assay was 10.94-700.00 μg·L-1 for osthol and 15.63-1 000.00 μg·L-1 for imperatorin(r=0.999 8,0.999 9,respectively;all P<0.05).The minimum quantifiable concentrations of osthol and imperatorin were 10.94 and 15.63 μg·L-1,respectively(s/n>4).The osthol and imperatorin of the extraction recoveries of low,medium and high concentration were 98.96%,95.30%,94.88% and 98.23%,98.94%,97.99% and RSD were less than 15%.Conclusion The established HPLC method is simple,sensitive,rapid and reliable for detection of osthol and imperatorin in rat blood drug level concentrations.更多还原