【摘要】 为探明猪FBXL8基因的分子结构特征,本试验以人FBXL8基因的mRNA序列作为信息探针,搜集猪同源基因EST序列,并构建EST重叠群Contig,设计出合适的引物,以湖北通城、大白和长白猪基因组为模板,应用PCR技术克隆测定FBXL8基因的第二内含子序列。序列分析结果表明猪FBXL8基因第二内含子为855 bp。通过运用SEQMAN软件对3个品种的序列比较分析,初步发现第二内含子的第325、361和493位碱基为SNP位点。更多还原

【Abstract】 In order to explore the molecular structural feature of porcine FBXL8 gene,the Homo FBXL8 mRNA sequence was used as an information probe to search for the porcine homologous ESTs sequence in this experiment,and the ESTs contig was constructed.Then the primers were designed according to it.Tongchen,Landrace and large white breeds DNA were used as the genomic template to amplify the second intron of porcine FBXL8 gene by PCR technique.The sequence analysis result indicates that it is a sequence of 855 bp long.Moreover,SEQMAN software was used to compare and analyze these sequences,and it was preliminarily found that there are three SNPs in the 325th,361th and 493th site respectively.更多还原