【摘要】 目的探查干扰素λ诱导人食管癌YES5和T.Tn细胞抗增殖的机制。方法流式细胞术检测细胞周期,膜联蛋白Ⅴ(annexinⅤ)和PI双染检测凋亡,Western印迹检测凋亡蛋白的表达。结果干扰素λ诱导人食管癌YES5和T.Tn细胞的亚G1期(sub-G1)升高,干扰素λ处理细胞与膜联蛋白Ⅴ的结合增加,干扰素λ可诱导细胞内半胱天冬酶(caspase)3的剪切,caspase抑制剂可减少干扰素λ诱导的sub-G1期升高。结论干扰素λ可诱导人食管癌YES5和T.Tn细胞发生凋亡。更多还原

【Abstract】 ObjectiveTo investigate the mechanism of growth inhibition of IFN-lambda(IFNλ) in human esophageal carcinoma YES5 and T.Tn cells.MethodsThe profiles of the cell cycle were analyzed with flow cytometer.Staining with annexin and PI was used for testing apoptosis.The intracellular protein was detected with Western blotting analysis.Results IFNλ enhanced the sub-G1 and annexin Ⅴ+ population in YES5 and T.Tn cells.IFNλ cleaved the caspase3.Caspase inhibitor eliminated the increased sub-G1 population induced by IFNλ.ConclusionIFNλ induces apoptosis in human esophageal carcinoma YES5 and T.Tn cells.更多还原