【摘要】 利用16对家猫微卫星引物和4对苏门答腊虎微卫星引物,对东北虎基因组DNA进行扩增,其中15对引物可以稳定扩增。这15个位点的等位基因数为3～7个,共检测到83个等位基因,平均等位基因数为5.53个。期望杂合度为0.585 0～0.841 3,平均为0.750 4;多态信息含量为0.512 9～0.821 7,平均为0.714 0。表明这15个微卫星位点在研究群体中均为高度多态性位点。运用SPSS11.5软件对20只东北虎个体间的亲缘关系进行了聚类分析,结果表明,个体间的亲缘关系清晰可见,除个别虎外,与系谱资料基本一致,在距离系数为5.988的水平上可聚为3类,A、B与C聚为1类,E、F与G聚为1类,D单独聚为1类。更多还原

【Abstract】 Sixteen microsatellite primers in the domestic cat(Felis catus) and four in the Panthera tigris sumatrae were tested in the P.t.altaica genome.15 of those 20 pairs of microsatellite primers can be successfully amplified.Totally 83 alleles were found,and the number were ranged from 3 to 7 for each locus.The average number of alleles was 5.53.The gene heterozygosity ranged from 0.585 0 to 0.841 3,and FCA077 and Pti003 had the highest and lowest value,respectively;the polymorphism information content ranged from 0.512 9 to 0.821 7,and FCA077 and Pti003 had the highest and lowest value respectively,which implied that the 15 microsatellite loci had high polymorphism in these P.t.altaica.The cluster analysis for 20 tigers was carried out with SPSS11.5 software and the 20 tigers were divided into three categories at 5.988.Except for few tigers,the blood relationship among the individuals was clear.The three categories were(A,B,C),(D),and(E,F,G).更多还原