【摘要】 目的探讨N-乙酰-L-色氨酸(L-NAT)对新生鼠脑缺血/缺氧损伤的保护作用。方法出生第7天的沙土鼠90只,随机分为正常对照组、缺血/缺氧组和L-NAT组。制备脑缺血/缺氧损伤模型,L-NAT组在造模前30min腹腔注射L-NAT(5mg/kg)。用Fluoro-Jade B(FJB)染色法观察细胞的死亡情况;用免疫荧光染色、Westernblotting观察活化的Caspase-3和Caspase-9在各组的表达。结果 L-NAT可使缺血/缺氧损伤后FJB阳性细胞数量减少,并抑制缺血/缺氧后Caspase-3和Caspase-9的活化。结论 L-NAT对新生鼠脑缺血/缺氧损伤动物模型具有保护作用。更多还原

【Abstract】 Objective To investigate the possible neuroprotective effects of N-acetyl-L-tryptophan(L-NAT) in cerebral ischemia/hypoxia injury.Methods The ischemia/hypoxia injury models were made in 90 pups of seven-days old gerbil.The death of neurons was evaluated by Fluoro-Jade B(FJB)staining and the activations of Caspase-9 and Caspase-3 were evaluated by immunofluorescent staining and Western blotting.Results Our results showed that L-NAT treatment decreased the number of FJB positive cells after middle cerebral artery occlusion in mice.And L-NAT also inhibited the expressions of Caspase-3 and Caspase-9.Conclusion Our work demonstrated that L-NAT had protective effect on cerebral ischemia/hypoxia injury.更多还原