【摘要】 目的探讨Wnt2b/β-catenin/C-myc信号途径在小鼠胚胎心脏发育中的时空表达。方法正常孕10d、12.5d、15d、17d、18.5d及出生后当天的小鼠胚胎心脏,免疫组织化学、RT-PCR和Western blotting等方法检测Wnt2b、β-catenin及C-myc在小鼠胚胎心脏发育不同时期的表达。结果 Wnt2b、β-catenin和C-myc的mRNA及蛋白量随胚胎发育时间的推移表达量逐渐降低;并且Wnt2b、β-catenin和C-myc的mRNA水平两两相关(P<0.05)。结论 Wnt2b/β-catenin/C-myc途径在小鼠胚胎心脏发育过程中发挥着重要作用;C-myc可能是与Wnt2b表达相关的下游靶基因之一。更多还原

【Abstract】 Objective To investigate the temporal and spatial expression of Wnt2b/β-catenin/C-myc signaling pathway in mouse embryonic heart.Methods The mouse embryonic hearts taken from gravidity 10 days(G10d),G12.5d,G15d,G17d,G18.5d,and postnatal day(P0d) were used.Immunohistochemistry,RT-PCR and Western blotting were performed to detect the expressions of Wnt2b,β-catenin and C-myc during different periods of mouse embryonic heart development.Results With the progress of the embryonic heart development,the expressions of Wnt2b,β-catenin and C-myc at the mRNA and protein levels were decreasing gradually,and the mRNA expression of Wnt2b,β-catenin and C-myc showed that they were interrelated statistically(P < 0.05).Conclusion These results suggest that Wnt2b/β-catenin/C-myc signaling pathway play important roles in regulating the development of the embryonic heart.And it can predict that C-myc might be one of Wnt2b downstream target genes.更多还原