【摘要】 目的探讨亚硒酸钠对人胃癌SGC-7901细胞系增殖和hTERT表达的作用及其机制。方法用含亚硒酸钠(0μmol/L、0.5μmol/L、2.5μmol/L、5μmol/L、8μmol/L)的培养液培养SGC-7901细胞24h、48h、72h、96h,通过四甲基偶氮唑盐(MTT)比色法检测亚硒酸钠对SGC-7901的增殖的影响,免疫细胞化学SABC法和RT-PCR检测亚硒酸钠对人胃癌细胞系SGC-7901hTERT蛋白和hTERTmRNA表达的作用。结果亚硒酸钠对人胃癌SGC-7901细胞具有明显的抑制作用,且随亚硒酸钠浓度的增加和培养时间的延长对SGC-7901细胞生长抑制作用逐渐加强。SGC-7901细胞hTERT蛋白免疫细胞化学染色的平均吸光度(AA),随0.5μmol/L、2.5μmol/L亚硒酸钠组培养时间增加逐渐降低,96h时明显低于空白对照组(P<0.01),5μmol/L、8μmol/L亚硒酸钠组的AA每个时间点均比空白对照组明显降低(P<0.01)。0.5μmol/L亚硒酸钠组SGC-7901 hTERTmRNA的表达无明显的变化;在2.5μmol/L亚硒酸钠组则随时间增加逐渐降低,48h后明显低于空白对照组;5μmol/L、8μmol/L亚硒酸钠组均明显低于空白对照组(P<0.01),72h后明显低于其他低硒组。结论亚硒酸钠抑制人胃癌SGC-7901细胞生长,下调hTERT表达。更多还原

【Abstract】 Objective To investigate effects of sodium selenite on human gastric cancer SGC-7901 cell growth and hTERT expression and to explore the mechanism.Methods SGC-7901 cells were treated with different concentrations(0μmol/L,0.5μmol/L,2.5μmol/L,5μmol/L,8μmol/L) of sodium selenite for 24 hours,48 hours,72 hours and 96 hours.Growth inhibition of SGC-7901 cells was detected by assay of MTT.Immunohistochemical SABC method and RT-PCR assay were used to study the changes of hTERT proteins and hTERT mRNA in SGC-7901 cells.Results Sodium selenite significantly suppressed the growth of SGC-7901 cells.The cell growth rate reduced in a dose-and time-dependent manner.The same changes had occurred for the hTERT protein level in SGC-7901 cells.The averae absorbance(AA) of hTERT protein in SGC-7901 cells was gradually lower along time,unto 96 hour it was significantly decreased in 0.5μmol/ L sodium selenite group or 2.5μmol/L sodium selenite group than that in blank control group(P < 0.01).It was significantly decreased in 5μmol/L,8μmol/L sodium selenite group than in blank control group all time(P < 0.01).RT-PCR results demonstrated there was no obvious alteration of hTERT mRNA levels after 0.5μmol/L sodium selenite treatment.hTERT mRNA levels were gradually reduced along time in 2.5μmol/L sodium selenite group,there was significantly different after 48 hours treatment in 2.5μmol/L sodium selenite group than in blank control group.hTERT mRNA levels were significantly decreased all time in 5μmol/L,8μmol/L sodium selenite group than in blank control group(P < 0.01),unto 72 hours it was significantly decreased than others lower sodium selenite groups(P < 0.05).Conclusion Sodium selenite suppressed the SGC-7901 cell growth,and down-regulated the hTERT expression.更多还原