【摘要】 目的:探讨正常妊娠ABCB1基因多态性与母胎界面皮质醇转运的关系。方法:用PCR-RFLP方法检测正常妊娠262例脐血及142例母血ABCB1 C3435T位点基因多态性,用化学发光法测定脐血及母血血清中皮质醇浓度。结果:ABCB1/C3435T基因多态性CC、CT和TT基因型频率脐血组分别为32.44%、53.82%和13.74%;母血组分别为35.91%5、0.70%和13.38%,两组差异无显著性(P>0.05)。TT基因型组胎儿血清皮质醇浓度小于CT及CC+CT基因型组,差异具有显著性(P<0.05);TT基因型组母血比脐血血清皮质醇浓度比值大于CT及CC+CT基因型组,差异具有显著性(P<0.05)。多因素回归分析显示,胎儿ABCB1/C3435T基因型与胎儿血清皮质醇浓度呈负相关(R2=0.399,B=-33.47,P=0.003),与母血比脐血血清皮质醇浓度比值呈正相关(R2=0.260,B=0.589,P=0.021)。母体ABCB1/C3435T基因多态性与血清皮质醇浓度无相关关系。结论:正常妊娠胎儿ABCB1/C3435T基因多态性与母胎界面皮质醇转运相关。更多还原

【Abstract】 Aim: To explore the relationship between ABCB1 polymorphisms and the cortisol transportation in maternofetal interface during normal pregnancy. Methods: the ABCB1/C3435T genotype was examined in 262 fetal and 142 maternal blood samples during normal pregnancy by PCR-RFLP.Blood serum cortisol levels were measured by fluorescence polarization immunoassay. Results: The frequency of the gene polymorphisms of ABCB1/C3435T: In the fetal group,the frequency of CC,CT,TT genotype were 32.44%,53.82%,13.74%.Respectively,and in the maternal group,the frequency of CC,CT,TT genotype were 35.91%,50.70%,13.38%,respectively.There was no statistic difference between the two groups(P>0.05).The fetal blood serum cortisol in the TT genotype group was significantly lower than those in the CT and CC+CT genotype groups(P<0.05);The blood serum maternal/fetal cortisol ratio in the TT genotype group was significantly higher than those in the CT and CC+CT genotype groups(P<0.05).Multivariable regression analyses showed that the fetal ABCB1/C3435T genotype revealed a significantly negative correlation with fetal blood serum cortisol(R2=0.399,B=-33.47,P=0.003) and a positive correlation with blood serum maternal/fetal cortisol ratio(R2=0.260,B=0.589,P=0.021).The maternal ABCB1/ C3435T polymorphism assessed by comparable models showed no significant association with the blood serum cortisol level. Conclusion:The fetal ABCB1/C3435T polymorphisms is related to the cortisol transportation in maternofetal interface during normal pregnancy.更多还原