【摘要】 表达反义βAS的5个人参发根系在液体培养条件下生长30天后,齐墩果烷型人参皂苷R0含量比对照实验组均有所降低,幅度分别达13%～40%;达玛烷型人参皂苷Rb1、Rb2、Rc、Rd、Re、Rf和Rg1含量在5个发根系中均有所增加,其中发根系A19的上述单体皂苷含量总和比对照实验组提高了30%;培养过程中达玛烷型人参皂苷含量呈逐渐增加趋势。5个发根系的βAS酶活性比对照实验组均有所下降,最多下降30%,DAS酶活性被上调,尤其发根系A19的酶活性比对照实验组提高了20%;化学分析结果显示,5个发根系人参皂苷前体物2,3-氧化角鲨烯的含量比对照实验组均有所提高。更多还原

【Abstract】 Five Panax ginseng hairy root lines expressing antisense βAS were established.After 30 days of growth cultured in liquid,the levels of oleanane-type ginsenoside Ro in these antisense hairy root lines decreased by 13% to 40% compared with control line.While levels of dammarane-type ginsenosides(Rb1,Rb2,Rc,Rd,Re,Rf and Rg1) increased in all five antisense hairy root lines,of which line A19 exhibited the max levels of dammarane-type ginsenosides,30% higher than those of control line.It was observed that dammarane-type ginsenosides contents in these hairy root lines gradually increased during culture process.Additionally,relative to control lines,βAS enzyme activities of the antisense lines decreased up to 30%;while DAS enzyme activities were enhanced,especially in the antisense lines A19(20% increase).Furthermore,the antisense lines show higher level of ginsenoside precursor(2,3-oxidosqualene) than the control line.更多还原