【摘要】 目的检测新型支架材料——聚乳酸-羟基乙酸/磷酸三钙(PLGA/TCP)的细胞毒性。方法采用扫描电子显微镜观察PLGA/TCP支架材料的表面及内部结构,并选用小鼠L929细胞株,用MTT法体外检测PLGA/TCP复合体的细胞毒性,与已被美国FDA批准应用于临床的PLGA支架材料进行比较。结果扫描电镜下PLGA/TCP支架材料表面及内部呈均匀多孔状,孔隙分布较均匀,孔隙直径平约400μm,在大孔的周围布满了相互贯通的微孔,外形多为近似圆形,微孔径为3～5μm,孔隙间相互贯通,平均孔隙率为89.6%。MTT法检测显示,PLGA/TCP复合体支架材料细胞毒性为1级,可认为无细胞毒性。结论 PLGA/TCP复合支架材料具有较为均一且近似天然骨小梁的结构,无细胞毒性,符合生物材料应用的基本要求。更多还原

【Abstract】 Objective To detecte the cytotoxicity of the PLGA/TCP(poly(lactic-co-glycolic acid)/Tricalcium phosphate) composite that based on the precedent experiments conducted in Tsinghua University.Methods Compared with the PLGA scaffold material,observated the surface and interior structure of the PLGA/TCP scaffold material by SEM(scanning electron microscope),the surface and interior of PLGA/TCP scaffold material appeared to be homogeneous porous under SEM,with fairly even porosity distribution.The pore diameter was approximately 400μm.The interpenetrative micro-pores were scattered over bigger pores’ periphery with approximately circular contour and 3~5 μm in diameter.These pores were interpenetrative,the average factor of porosity was 89.6%.And which selected rat L929 cell strain,and detected the cytotoxicity of the PLGA/TCP composite in vitro by MTT method.Results The surface and interior of PLGA/TCP scaffold material appeared to be homogeneous porous under SEM,with fairly even porosity distribution.The pore diameter was approximately 400μm.The interpenetrative micro-pores were scattered over bigger pores’ periphery with approximately circular contour and 3~5 μm in diameter.These pores were interpenetrative,the average factor of porosity was 89.6%.On rat L929 cell strain,used MTT Method to detect the cytotoxicity of the composite PLGA/ TCP in vitro,the result showed that the cytotoxicity of the PLGA/TCP composite was level I,according to the criterion,it can be considered as non cytotoxic.Conclusion This research has proved that the PLGA/TCP compound scaffold material has a more homogeneous structure,with the vesicular interior and the structure of PLGA/TCP composite is similar to natural bone trabecula,PLGA/TCP is non cytotoxicity,which satisfy the basic requirement of biological material application and provides a good experimental foundation for repairing autologous bone defect in the near future.更多还原