【摘要】 目的探讨羧胺三唑(CAI)对BV2细胞模型中多种炎性细胞因子的调节作用。方法将不同浓度的CAI加入BV2细胞中培养18 h后,用CCK-8法测定其细胞数。分别用ELISA试剂盒和硝酸盐还原法测定细胞培养基上清中的TNF-α、IL-1β、IL-6、NO的含量,用荧光定量PCR法测定BV2细胞中TNF-α、IL-1β、COX-2和iNOS的mRNA的含量。结果与对照组相比,CAI浓度在20、30、40μmol/L能够剂量依赖性抑制BV2细胞TNF-α的分泌[(359.4±12.9)vs(241.6±16.1),(135.7±6.4),(5.3±1.9)pg/mL,P<0.01],也可显著抑制IL-1β、NO、IL-6的分泌(P<0.01),同时也能够降低TNF-α、IL-1β、COX-2和iNOS的mRNA的表达(P<0.01)。结论 CAI能够明显降低在ALS的BV2细胞模型中炎性介质TNF-α、IL-1β、NO、IL-6的分泌以及TNF-α、IL-1β、COX-2和iNOS的mRNA的表达,对小胶质细胞的活化及其炎性反应有明显的抑制作用。更多还原

【Abstract】 Objective To explore the regulation of carboxyamidotriazole(CAI) on inflammatory factors of BV2 cell model.Methods BV2 cells were incubated with different concentrations of CAI(5~40 μmol/L),LPS was then added and cells were incubated for 18 h,TNF-α,IL-1β,IL-6 levels were determined with ELISA kits and NO levels were determined with Griess reagent,respectively the mRNA levels of TNF-α,IL-1β,COX-2 and iNOS were detected by Real-time Quantitative PCR.Results The viability of BV2 cells was not affected at the concentrations of CAI used(5~40 μmol/L),CAI was found to reduce TNF-α production [(359.4±12.9) vs(241.6±16.1),(135.7±6.4),(5.3±1.9)pg/mL,P<0.01] and IL-1β,NO,IL-6 products(P<0.01) in a dose-depended manner at the concentrations 20,30 and 40μmol/L;CAI was also found to inhibit the mRNA expression of TNF-α,IL-1β,COX-2 and iNOS.Conclusion The present study suggests that CAI has obvious effect on TNF-α,IL-1β,NO,IL-6 secretions of BV2 cells model,and CAI was found to has a remarkable inhibitory effect on the activationand inflammatory response of the LPS-induced BV2 cells.更多还原