【摘要】 目的探讨IL-17与HBV感染特别是肝纤维化发生的相关性。方法酶联免疫吸附试验(ELISA)及反转录-多聚酶链反应(RT-PCR)法测定血清中IL-17浓度和外周血单个核细胞(PBMC)中IL-17mRNA的水平。免疫组化法及ELISA法测定肝组织中IL-17的表达和Ⅳ型胶原、层黏连蛋白(LN)、透明质酸(HA)及Ⅲ型胶原的水平。结果 4组患者中血清IL-17和PBMC中IL-17mRNA水平表达均高于正常对照组(P<0.005);与其他各组相比,IL-17及其mRNA水平在肝硬化患者中明显升高(P<0.005)。肝组织IL-17表达强度及血清肝纤维化指标水平在肝硬化组高于慢性乙肝组,慢性乙肝组高于携带者组(IL-17表达强度P<0.01,肝纤维化指标P<0.001);肝组织IL-17染色位于汇管区,与纤维化高度相关;淋巴细胞、成纤维细胞及血窦内皮细胞染色阳性。肝组织IL-17表达强度与血清Ⅳ型胶原、LN、HA、Ⅲ型胶原呈正相关(r=0.883、0.834、0.793、0.722,P<0.01)。结论 IL-17可能参与慢性HBV感染尤其是肝纤维化的发生过程。更多还原

【Abstract】 Objective To explore the role of interleukin-17(IL-17) in the pathogenesis and progress of chronic hepatitis B virus(HBV) infectious diseases.Methods Serum IL-17 level and liver fibrosis index type Ⅳ collagen,Laminin,hyaluronidase,type Ⅲ collagen were determined by ELISA.IL-17 mRNA expression in peripheral blood mononuclear cell(PBMC) was analysed by RT-PCR.IL-17 expression in liver was measured by immunohistochemistry.Results Serum IL-17 protein levels and IL-17 mRNA expression of PBMC in four HBV infectious groups(P<0.005) were higher than those in normal control.IL-17 and IL-17 mRNA in Liver cirthosdis was higher than that of other groups(P<0.005).Liver IL-17 expression and serum fibrosis index raised with severityin in group of Liver cirthosis higher than chronic heptitis B,while chronic heptitis B group was higher than asymptomatic HBV camer group(liver IL-17 expression P<0.01,serum fibrosis index P<0.001).IL-17 mainly located in portal area and was associated with fibrosis closely.Lymphocyte,fibroblast and sinusoid endotheliocyte were stained positively.Liver IL-17 expression was correlated with serum fibrosis index type Ⅳ collagen,LN,HA,type Ⅲ collagen positively(r=0.883,0.834,0.793,0.722,P<0.01).Conclusion IL-17 may contribute to the development of HBVinfectious disease especially to the pathophysiology of LC.更多还原